Preparation method of diphenyl oxide compound and application of the compound as natural marine organism antifouling agent
A technology of compound and diphenyl ether, which is applied in the preparation of diphenyl ether compound and as a natural marine biological antifouling agent, can solve the problems of marine biological antifouling agents that have not been seen yet, and achieve the effect of broad application prospects
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[0014] Preparation of Compounds of the Invention
[0015] (1) The medium used for the culture of the fungus Aspergillus sp. (HM535361) contains 1.0% glucose (percentage by weight, the same below), 0.1% yeast extract, 0.2% peptone, 1.0% agar, 3% coarse sea salt, and the rest is Water, made into a test tube slant when used, and the fungal strains were cultured at 28°C for 5 days.
[0016] The strain medium contains 0.1%-5.0% of glucose (percentage by weight, the same below), 0.01%-1% of yeast extract, 0.01%-1% of peptone, 0.1%-3.0% of agar, and 0.1%-5% of coarse sea salt , and the rest is water. The culture temperature is 5-45° C., and the culture time is 3-10 days.
[0017] (2) Fermentation culture of the fungus Aspergillus sp. (HM535361) The fermentation medium used was rice solid medium, and 100g of rice, 3g of coarse sea salt, 0.6g of peptone, and 100mL of water were added to each 1000mL Erlenmeyer flask. The fungal strains were grown at 28°C for 30 days.
[0018] The fe...
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