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Protein IbGGPS, encoding gene and application thereof to plant carotenoid content control

A technology of carotene and protein, applied in the fields of application, plant products, genetic engineering, etc., can solve problems such as difficult operation and poisoning

Inactive Publication Date: 2013-07-17
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Facing the world, especially some countries in Africa V A The lack of status quo has been suggested by taking V A resolved, but in the long run, taking V A The operation is difficult, and V A Excessive intake can cause poisoning, so intake of V from daily diet A The precursor β-carotene is an economically safe route

Method used

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  • Protein IbGGPS, encoding gene and application thereof to plant carotenoid content control
  • Protein IbGGPS, encoding gene and application thereof to plant carotenoid content control
  • Protein IbGGPS, encoding gene and application thereof to plant carotenoid content control

Examples

Experimental program
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Effect test

Embodiment 1

[0056] Example 1. Acquisition of proteins related to sweet potato geranylgeranyl pyrophosphate synthase and its coding genes

[0057] 1. Acquisition of proteins related to sweet potato geranylgeranyl pyrophosphate synthase and their coding genes

[0058] (1) Cloning of IbGGPS protein cDNA from sweet potato (Ipomoea batatas)

[0059] Experimental Materials:

[0060] Nongdafu 14 (high carotenoid content mutant of Gaoxi No. 14) (the public can obtain from China Agricultural University, the non-patent documents that have recorded Nongdafu 14 (Nongdafu14) are: Wang YP, Wang F, Zhai H, Liu QC. Production of a useful mutant by chronic irradiation in sweetpotato. Scientia Horticulturae111, 2007:173–178;) planted in the field for about 90 days, harvested tubers with a diameter of 3 to 4 cm in the early stage of root expansion, quick-frozen in liquid nitrogen, -80°C save.

[0061] 1. Extraction and purification of root tuber total RNA

[0062] Take about 2 g of the enlarged root tub...

Embodiment 2

[0083] Embodiment 2, the application of sweet potato IbGGPS protein in improving carotenoid content in plant tissue

[0084] 1. Acquisition of trans IbGGPS tobacco

[0085] 1. Construction of plant expression vectors

[0086] According to the coding sequence of the sweet potato IbGGPS protein cDNA, the primer sequences for amplifying the complete coding sequence were designed, and the forward and reverse primers were respectively introduced into the BamH I and Sac I restriction sites. The primer sequences were as follows:

[0087] Primer 7: 5'-G GGATCC ATGAGGTCGATGAATCTT-3' (sequence 5 in the sequence listing) (the underlined part is the BamH I restriction site),

[0088] Primer 8: 5′-TC GAGCTC TTAATTCTGTCTATAAGC-3' (sequence 6 in the sequence listing) (the underlined part is the Sac I restriction site).

[0089] Using the DNA molecule shown in Sequence 2 in the artificially synthesized sequence listing as a template (or using Nongdafu 14 root cDNA as a template), afte...

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Abstract

The invention discloses a protein IbGGPS, an encoding gene and an application thereof to plant carotenoid content control. The protein IbGGPS provided by the invention is obtained from Ipomoea batatas and is the protein of (a) or (b), wherein (a) is a protein which consists of an amino acid sequence shown in sequence 2 in a sequence table; and (b) is a protein which is formed by replacing and / or deleting and / or adding one or a plurality of amino acid residues on the amino acid sequence shown in sequence 2 in the sequence table and derived from sequence 2 which is related to the carotenoid content of plant tissues. Experiments prove that the alpha-carotenoid, beta-carotenoid and total carotenoid contents of tobacco can be improved through IbGGPS gene overexpression.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a protein IbGGPS and its encoding gene and its application in regulating plant carotenoid content. Background technique [0002] In nature, carotenoids widely exist in plants, fungi, algae, and bacteria, showing a color change from yellow to red. Animals cannot synthesize them by themselves and need to be ingested directly or indirectly from plants, fungi, and bacteria. Carotenoids are essential micronutrients for animals and humans. They can synthesize retinoids, scavenge free radicals, and prevent cancer, cardiovascular and senile diseases. The lack of carotenoids will seriously affect human health, night blindness is the lack of V A due to. Night blindness can be cured by taking an appropriate amount of β-carotene in the early stage. Facing the world, especially some countries in Africa V A The lack of status quo has been suggested by taking V A resolved, but in the long run,...

Claims

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Application Information

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IPC IPC(8): C12N9/10C12N15/54C12N15/82C12N5/10C12N1/15C12N1/19C12N1/21C12N15/11A01H5/00
Inventor 刘庆昌翟红何绍贞陈伟
Owner CHINA AGRI UNIV
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