cyp450 genotype database and method for genotyping and enzyme activity identification

Abstract

The invention discloses a method for constructing a CYP450 gene standard type database, which comprises first comparing the specific sequence corresponding to the mutation information of the CYP450 genotype with the standard sequence of the whole human genome to obtain the correspondence between the standard sequence and the standard sequence of the whole human genome relationship; then according to the corresponding relationship, the CYP450 genotype is converted into a genotype with the standard sequence of the whole human genome as the reference sequence. The invention also discloses a database constructed by the above method, and a CYP450 genotyping and enzyme activity identification method based on the database. The database of the present invention facilitates the research of CYP450, and the genotyping method provides a unified standard for CYP450 genotyping, and provides more accurate judgment basis for diseases or drugs; it can effectively detect unknown mutation sites in CYP450 genes ; It can detect up to hundreds of samples at the same time; the coverage is comprehensive and extensive.

Description

Technical field [0001] The present invention relates to the field of gene detection, in particular to a CYP450 standard genotype database, as well as a CYP450 genotyping method and an enzyme activity identification method. Background technique [0002] Cytochrome P450 is abbreviated as CYP450. At present, 57 CYP450 oxidases have been identified from the human body. Among them, the drug metabolism is mainly concentrated in the CYP1, CYP2 and CYP3 families, and it is involved in the metabolism of more than 90% of the current drugs. The important role of CYP450 in drug metabolism has led to CYP450 gene polymorphisms becoming one of the most important factors affecting individual drug differences. Its polymorphisms include point mutations, insertions or deletions, deletions or duplications of entire genes, and ultimately lead to enzyme activity Enhanced, weakened, or completely absent. CYP450 polymorphisms can cause serious side effects or ineffectiveness in individuals under standa...

AI Technical Summary

Problems solved by technology

[0005] However, the current technology for CYP450 mutation detection mainly focuses on the known mutations of single or several P450 genes; and, the mutation sites in each study are relative to their specific sequences, and there is no unified measurement and comparison between them standard

In addition, the existing CYP450 detection methods are not efficient in detecting unknown mutation sites that still exist in the CYP450 gene; when a large number of samples need to be detected, it takes a long time and cannot well meet the needs of research and practical applications

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