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Tumour cell migration capability evaluation method and application

A technology of tumor cells and metastasis ability, applied in the field of tumor cell metastasis ability evaluation, can solve the problems of inconvenient test methods, inaccurate test results, only considering a single molecule, etc., to shorten the development cycle, shorten the development cycle, and easily judge the results. Effect

Inactive Publication Date: 2013-07-03
SHANDONG XINBO PHARMA R&D
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Problems solved by technology

In vitro models mainly focus on the mechanism research of a single molecule and the detection of invasion and metastasis ability. The existing test methods are not only inconvenient, but also only consider a single molecule, resulting in inaccurate test results
[0004] The research and development of new drugs in any country is a difficult process with high investment, high risk and time-consuming, but once successful, there will be huge benefits

Method used

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  • Tumour cell migration capability evaluation method and application
  • Tumour cell migration capability evaluation method and application
  • Tumour cell migration capability evaluation method and application

Examples

Experimental program
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Effect test

Embodiment 1

[0039] Example 1 Screening Tumor Cells with Strong Metastatic Ability Using Tumor Cell Metastatic Ability Evaluation Method

[0040] Evaluate the metastatic ability of tumor cells Colo205 and CaCo2, including the following steps:

[0041] (1) Collection and culture of tumor cells:

[0042] Take Colo205 and CaCo2 cells for monoclonalization, stimulate the monoclonal cells with 10M Staurosporin to induce recovery of cell connection, select the clone with the largest change in cell area before and after stimulation as the experimental object, and prepare cell suspension; the following method can also be used to collect Tumor cells: Take the tumor cells to be evaluated for monoclonalization and make a cell suspension; or, take the tumor cells to be evaluated for monoclonalization to obtain monoclonal cells of the tumor cells to be evaluated, and use Staurosporin to stimulate and induce the monoclonal cells To restore cell connection between monoclonal cells, select monoclonal cel...

Embodiment 2

[0059] Example 2 Using the colorectal cancer epithelial cells Colo205 cells screened in Example 1 to screen anti-metastatic drugs

[0060] The Colo205 cells were monoclonalized, and the monoclonal cells were stimulated with 10M Staurosporin to induce recovery of cell connection, and the clone with the largest change in cell area before and after stimulation was selected as the experimental object.

[0061] The Opera drug screening system, a high-throughput and high-content screening system of PE Company, is used to put Colo205 into the test plate running candidate drugs in 96, 384 or 1536 microwell plates, which can realize automatic drug addition, plate operation, pipetting operation and Incubators for various applications in live cells, compound delivery / transfer, plate fixation and washing for fixed cell assays. Transfer the microplate to the Opera system, use pre-set measurement and analysis parameters for fully automated screening, and analyze images online in real time. ...

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Abstract

The invention provides a tumour cell migration capability evaluation method. The tumour cell migration capability evaluation method comprises the following steps of: collecting and culturing tumour cells, namely collecting tumour cells to be evaluated to obtain cell suspension, and culturing the cell suspension; and evaluating tumour cell migration capability, namely injecting a tumour cell anti-migration drug, and then detecting cell morphology, cell layer area, connecting strength between cells and a polyacrylamide culture substrate and connecting strength among cells as tumour cell migration capability evaluation criterions. The tumour cell migration capability evaluation method provided by the invention has the characteristics of low cost, easy operation, easily discriminated result and high speed and accuracy. By utilizing the tumour cell migration capability evaluation method, molecular biology and cell biology means are taken as basis, cancer cells with strong transitivity are screened, an in vitro cancer cell migration model is built, drugs with potential inhibiting effect on tumour cell migration can be effectively screened, a simple and practicable method is provided for high-throughput drug screening, and a development period of a new drug is shortened.

Description

technical field [0001] The invention belongs to the field of biomedicine, in particular to a method for evaluating tumor cell metastasis ability. Background technique [0002] In the past few decades, the screening of antineoplastic drugs was mainly to investigate their toxic effects on fast-growing cells. With the development of tumor biology research, it is recognized that tumor cells not only have strong proliferation ability, but also have the ability to destroy their own tissues, infiltrate adjacent tissues, infiltrate blood vessels or lymphatic vessels, and transfer to other tissues along with blood or lymph. Ability. The interaction between tumor and normal tissue has a great relationship with the characteristics of tumor angiogenesis, invasion and metastasis. [0003] Tumor metastasis has become one of the research hotspots in recent years. Existing studies on tumor metastasis mainly include in vitro or in vivo models. The in vivo models are mainly studied with im...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/02C12N5/09
Inventor 孟文翔滕文臣
Owner SHANDONG XINBO PHARMA R&D
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