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Sodium alginate containing high molecular weight polyguluronic acid and application thereof

A technology of polyguluronic acid and high molecular weight, applied in the direction of being fixed on/in an organic carrier, can solve the problems of insufficient guluronic acid content, low mechanical strength, low molecular weight, etc. Diffusion and transfer, large molecular weight, good biocompatibility

Active Publication Date: 2013-07-03
青岛聚大洋藻业集团有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Among many encapsulation technologies, capsules prepared with alginate (alginate) have been widely used due to their advantages of simple preparation, easy scale-up, and good cell activity. However, there are still some problems in the alginate immobilization technology, such as Insufficient guluronic acid content and low molecular weight in alginate will result in low mechanical strength and high shrinkage defects, especially in large-sized capsules

Method used

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  • Sodium alginate containing high molecular weight polyguluronic acid and application thereof
  • Sodium alginate containing high molecular weight polyguluronic acid and application thereof
  • Sodium alginate containing high molecular weight polyguluronic acid and application thereof

Examples

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Embodiment 1

[0038] The stems and leaves of mature Chilean giant algae (Lessonia trabeculata) were selected and dried, crushed in a pulverizer, and passed through a 20-mesh sieve to obtain algae powder. Take 100g of crushed algae powder, mix the algae powder with 80wt% ethanol at a ratio of 1:10 (W / V, g / ml), then reflux and stir in a water bath at 80°C for 2 hours, discard the upper layer, and repeat the degreasing 2 times, dry the defatted algae powder. Mix the dry defatted algae powder with water at a ratio of 1:20 (W / V, g / ml), stir and extract at 80°C for 2 hours, discard the supernatant, repeat the extraction twice, and collect the algal residue by filtration. Add 2wt% sodium carbonate aqueous solution with a solid-liquid ratio of 1:30 (W / V, g / ml) to the algae residue after hot water extraction, extract at 85°C for 3 hours, centrifuge to take the supernatant, and repeat the extraction 3 times , and pool the supernatants. The supernatant was treated with 2mol·L -1After neutralizing w...

Embodiment 2

[0041] The stems and leaves of mature Chilean giant algae (Lessonia trabeculata) were selected and dried, crushed in a pulverizer, and passed through a 60-mesh sieve to obtain algae powder. Take 100g of crushed algae powder, mix the algae powder with 85wt% methanol at a ratio of 1:20 (W / V, g / ml), then reflux and stir in a water bath at 85°C for 2 hours, discard the upper layer, and repeat the degreasing 2 times, dry the defatted algae powder. Mix the dry defatted algae powder with water at a ratio of 1:20 (W / V, g / ml), stir and extract at 85°C for 3 hours, discard the supernatant, repeat the extraction twice, and collect the algal residue by filtration. Add 10wt% ammonium carbonate aqueous solution with a solid-liquid ratio of 1:20 (W / V, g / ml) to the algae residue after hot water extraction, extract at 85°C for 2 hours, centrifuge to take the supernatant, and repeat the extraction 3 times , and pool the supernatants. The supernatant was treated with 2mol·L -1 After neutraliz...

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Abstract

The invention relates to the field of sodium alginate, in particular to sodium alginate containing high molecular weight (HMW) a-1, 4-L-polyguluronic acid, and an application thereof in immobilized cells and microorganisms. Stems and leaves of ripe kelp are taken as raw materials, the raw materials are crushed and then subjected to organic solvent degreasing and hot water extraction, and residues are collected; the residues are subjected to thermokalite extraction and then filtered, supernatant fluid is collected and subjected to hydrochloric acid precipitation, neutralization, dehydration and drying to obtain the sodium alginate containing HMW high polyguluronic acid content, in which the content of polyguluronic acid is larger than or equal to 90 mol%, and the molecular weight is larger than or equal to 70 kD. The sodium alginate has the advantages of rich raw material sources, good safety, unique structure, large molecular weight, high gel strength and simple preparation technology. A manufactured microcapsule is 0.5 mm-5.0 mm in particle size, so that large-scale preparation and product reclamation are facilitated; and the microcapsule is manufactured under physiological conditions, so that physiological activities of cells and microorganisms are guaranteed.

Description

technical field [0001] The invention relates to the field of alginate, in particular to an alginate containing high molecular weight α-1,4-L-polyguluronic acid and its application in immobilized cells and microorganisms. Background technique [0002] Alginate is composed of different proportions of mannuronic acid (M) and guluronic acid (G). Different brown algae and different parts of the same brown algae have different structures of alginate, and different structures of alginate have different physical and chemical properties. , so that this type of polysaccharide is widely used in the fields of food, medicine and chemical industry. The methods for preparing alginate from brown algae mainly include physical methods, chemical methods and enzymatic methods, among which: physical methods mainly include water extraction, chemical methods mainly include alkali extraction and acid precipitation, and enzymatic methods mainly use various brown algae. gelase for extraction. A lar...

Claims

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Application Information

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IPC IPC(8): C08B37/04C12N11/10C12N11/04
Inventor 于广利孙学超刘亚男吴建东从大鹏单鑫迪
Owner 青岛聚大洋藻业集团有限公司
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