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Glycyrrhetinic acid nano-liposome for improving immune function of livestock and poultry and its preparation method

A nano-liposome and glycyrrhetinic acid technology, which is applied in liposome delivery, medical preparations with non-active ingredients, medical preparations containing active ingredients, etc., can solve the problem of low water solubility of glycyrrhetinic acid and easy to cause adverse reactions Response, low bioavailability and other problems, to improve animal cellular immune function and humoral immune function, enhance animal humoral immunity and cellular immunity, and prolong the effect of the drug

Inactive Publication Date: 2013-07-03
NANJING AGRICULTURAL UNIVERSITY
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Problems solved by technology

[0006] Technical Problems The present invention aims at the problems of low water solubility of glycyrrhetinic acid, difficult to be absorbed, easy to produce adverse reactions, low bioavailability, etc., and provides a preparation method of glycyrrhetinic acid nano-liposomes, which can significantly improve the immune enhancement of glycyrrhetinic acid The purpose of action and clinical application in livestock and poultry

Method used

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  • Glycyrrhetinic acid nano-liposome for improving immune function of livestock and poultry and its preparation method
  • Glycyrrhetinic acid nano-liposome for improving immune function of livestock and poultry and its preparation method
  • Glycyrrhetinic acid nano-liposome for improving immune function of livestock and poultry and its preparation method

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Embodiment Construction

[0017] 1. Preparation of Glycyrrhetinic Acid Nanoliposomes

[0018] Glycyrrhetinic acid liposomes were prepared by film dispersion method. Accurately weigh soybean lecithin, cholesterol, Tween-80 and glycyrrhetinic acid, add 10 mL of chloroform and shake to fully dissolve them. Evaporate under reduced pressure on a rotary evaporator to remove chloroform, so that the solution forms a uniform film on the wall of the bottle, then add phosphate buffer solution (PBS, pH 7.4), continue to spin and elute until the film is completely dissolved, and then ultrasonicate for 20 minutes in a water bath. Nano liposomes are formed, and then passed through a microporous membrane with a pore size of 0.22 μm. The liposomes were frozen at -20°C and then thawed in a water bath, and this operation was repeated twice to increase the encapsulation efficiency.

[0019] On the basis of the single factor test, select three factors that have a more significant impact on the liposome encapsulation effi...

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Abstract

Belonging to the fields of preparation technologies and immune application of herbal veterinary drugs, the invention relates to a glycyrrhetinic acid nano-liposome for improving the immune function of livestock and poultry and its preparation method. A film dispersion technique is employed to prepare the glycyrrhetinic acid nano-liposome. With the encapsulation rate as an index, a response surface technique is adopted to study the following 3 factors: a lipid-drug ratio, a film material ratio and a water bath temperature, and preferable selection is conducted to determine the optimal preparation conditions as: a lipid-drug ratio of 9.0:1, a film material ratio of 2.5:1, and a water bath temperature of 31DEG C. The obtained glycyrrhetinic acid nano-liposome has a high encapsulation rate and strong immunoenhancement activity. The glycyrrhetinic acid nano-liposome provided in the invention significantly enhances the immunoenhancement activity of glycyrrhetinic acid.

Description

1. Technical field [0001] The invention relates to a preparation method of glycyrrhetinic acid nano-liposome and its application for improving the immune function of livestock and poultry, and belongs to the field of Chinese veterinary medicine preparation technology and immune application. 2. Background technology [0002] Licorice is the most commonly used Qi-invigorating medicine. It has the functions of invigorating the spleen and Qi, clearing away heat and detoxification, eliminating phlegm and relieving cough, relieving spasms and relieving pain, and harmonizing various medicines. It plays an important role in the prevention and treatment of various diseases. Glycyrrhetinic acid, as the main active ingredient of licorice, is mainly extracted from the roots or rhizomes of licorice, and belongs to pentacyclic triterpene saponins. Modern studies have shown that glycyrrhetinic acid has various pharmacological effects such as anti-tumor, anti-inflammatory, anti-virus, anti-...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K9/127A61K31/56A61K47/34A61P37/04
Inventor 王德云赵晓娟胡元亮刘家国范云鹏袁菊
Owner NANJING AGRICULTURAL UNIVERSITY
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