Yili Horse Breeding Method and Its Kit
A kit and female parent technology, applied in the field of breeding methods and kits for improving the body size traits of Yili horses, can solve the problems of low horse heritability, long generation interval, and difficulty in obtaining genetic progress, and achieve accelerated breeding and improved Effects of body size traits
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[0013] 1. Acquisition of SNPs related to body size traits of Yili horses
[0014] (1) Extraction of Yili horse genome DNA
[0015] Collect Yili horse blood, use heparin sodium anticoagulant, use Tiangen Blood Genome Kit to extract Yili horse genomic DNA, detect by 0.8% agarose gel electrophoresis, measure its concentration and purity with a nucleic acid protein quantifier, dilute to 50ng / ul, and store spare. (2) Amplification of the target fragment
[0016] Primers were designed according to the genome sequence of the horse included in the NCBI database, including the forward primer F: 5'-TGGAGTCAGTTGGGTTTAATG-3' and the reverse primer R: 5'-GACCGGATAGCATAGAGAGAG-3' to amplify the target fragment. As shown in SEQ ID NO.1, the SNP is located at 170 bp amplified to the target fragment, and the base at this position can be C or T. When the base at this position is C, it can be recognized by the Alu I endonuclease, and the recognition The sequence is AGCT.
[0017] The PCR rea...
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