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Petroleum hydrocarbon degradation bacteria being suitable for electric field condition and application thereof

A technology for petroleum hydrocarbon degrading bacteria and petroleum hydrocarbons, applied in the field of environmental biology, to achieve high activity, enhanced degradation activity, and good economic benefits

Active Publication Date: 2013-06-19
SHENYANG INST OF APPLIED ECOLOGY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to provide a kind of petroleum hydrocarbon degrading bacteria suitable for electric field conditions and its application in view of the problems existing in the electric-microbial remediation technology of petroleum-contaminated soil

Method used

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  • Petroleum hydrocarbon degradation bacteria being suitable for electric field condition and application thereof
  • Petroleum hydrocarbon degradation bacteria being suitable for electric field condition and application thereof
  • Petroleum hydrocarbon degradation bacteria being suitable for electric field condition and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Example 1: Isolation and screening of Bacillus licheniformis PS5 CCTCC No: M2011261

[0022] The sample comes from the petroleum hydrocarbon-contaminated soil around the Liaohe Oilfield. Weigh 5g of soil, add 50ml of sterile water to shake for 30 minutes, and then draw the supernatant to the enrichment medium containing 1000mg / L petroleum hydrocarbon, that is, the inorganic salt plus petroleum hydrocarbon medium ( Composition of medium: MgSO 4 .7H 2 O 2.6g; CaCl 2 .H 2 O 0.16g; K 2 HPO 4 6.0g; KH 2 PO 4 6.0g; (NH 4 ) 2 SO 4 6.0g; NaCl 12.0g; distilled water 1000ml; pH 7.2-7.4, sterilized at 121°C for 30min), after culturing for 3 days, streak and isolate on a solid inorganic salt medium containing petroleum hydrocarbons, and select a single colony with good growth to streak and isolate After three purifications, the strains were maintained on slants.

[0023] Inoculate the obtained pure strains in the liquid inorganic salt medium containing three petroleum hy...

Embodiment 2

[0025] Example 2: Biological activity of Bacillus licheniformis PS5 CCTCC No: M2011261 under electric field conditions

[0026] The above-mentioned Bacillus licheniformis (Bacillus licheniformis) PS5 was inoculated in beef extract peptone liquid medium, and then cultured on a constant temperature shaker at 30°C and 160r / min for 3 days; Buffer diluent (stock solution KH 2 PO 434.0g; 175ml of 1mol / L NaOH solution; 825ml of distilled water; pH7.2; diluent: take 1.25ml of the stock solution, dilute to 1000ml with distilled water, and autoclave at 121°C for 30min) to prepare the bacteria into a suspension. The prepared bacterial suspension of Bacillus licheniformis (Bacillus licheniformis) PS5 was added to the sterilized clean meadow brown soil (soil of Shenyang Ecological Station, Chinese Academy of Sciences), and the concentration of Bacillus licheniformis in the soil was 6.8×10 7 CFU / g dry soil. At the same time, unidirectional and switching electric fields were applied in th...

Embodiment 3

[0028] Example 3: Degradation of alkanes, aromatics and colloids by Bacillus licheniformis PS5 CCTCC No: M2011261 under different electric field conditions

[0029] Bacillus licheniformis (Bacillus licheniformis) PS5 was inoculated in beef extract peptone liquid medium, and then cultured on a constant temperature shaker at 30°C and 160r / min for 3 days; the bacteria were collected by centrifugation at 8000r / min at room temperature, and then buffered with phosphate buffer. Prepare the bacterial cells into a bacterial suspension. The prepared bacterial suspension was inoculated respectively in contaminated soil containing alkanes (550mg / kg), aromatics (350mg / kg), colloids (200mg / kg), and the concentration of Bacillus licheniformis in the soil was 6.8×10 7 CFU / g dry soil. At the same time, unidirectional and switching electric fields were applied in the test soil, the voltages were 0.5V / cm, 1.0V / cm, 2.0V / cm, the electrode spacing was 24cm, the soil humidity was 25%, and the exper...

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Abstract

The present invention discloses petroleum hydrocarbon degradation bacteria being suitable for electric field condition and application thereof. Bacillus licheniformis PS5 of the present invention is isolated from petroleum contaminated soil, can degrade petroleum hydrocarbons, and is preserved in the China Type Culture Collection center on July 19, 2011, wherein the preservation registration number is CCTCC No: M 2011261. The bacillus licheniformis PS5 is used for the remediation of contaminated soil. The strain isolated by the invention has high activity under the condition of 0.5-2.0 V / cm field, and can improve removal of alkanes, aromatics, colloids and mixed petroleum hydrocarbon. By applying the electric field, the degradation activity of the bacillus licheniformis PS5 is increased. After 28 days the removal rates for petroleum hydrocarbon single components and mixed petroleum hydrocarbons are increased by 4.19-21.91% and 7.64-23.32%.

Description

technical field [0001] The invention relates to the technical field of environmental biology, in particular to a petroleum hydrocarbon degrading bacterium suitable for electric field conditions and its application. Background technique [0002] With the increase of human demand for oil, the ways and chances of oil leakage increase, and oil has become one of the bulk pollutants in the soil. The composition of petroleum is complex, containing organic substances such as saturated hydrocarbons and aromatic hydrocarbons. After these substances enter the soil, they are first adsorbed on the surface of the macropores of the soil and various organic and inorganic particles, and then gradually diffuse into the micropores of the soil or distribute into the soil organic matter. Inside, a small part exists as pure solid or non-aqueous phase substances. When the infiltration of oil exceeds the self-purification capacity of the soil, the accumulated oil will remain for a long time, destr...

Claims

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Application Information

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IPC IPC(8): C12N1/20B09C1/10A62D3/02C12R1/10A62D101/20
Inventor 郭书海李凤梅张玲妍李刚李婷婷
Owner SHENYANG INST OF APPLIED ECOLOGY - CHINESE ACAD OF SCI
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