Kit and preparation method for identifying and detecting a variety of sheep Theileria

Abstract

The invention discloses a multiple PCR (Polymerase Chain Reaction) identification kit for detecting and identifying sheep Loeffler theileria, sheep Dyen theileria and sheep Theileria. The kit for identifying and detecting various sheep theileria comprises three pairs of specific primers, which are respectively upstream and downstream primers of sheep Loeffler theileria, upstream and downstream primers of sheep Dyen theileria, and upstream and downstream primers of sheep theileria. In the specific embodiment of the invention, the primers in the kit for identifying and detecting various sheep theileria are respectively SEQ ID NO. 1, SEQ ID NO. 2, SEQ ID NO. 3, SEQ ID NO. 4, SEQ ID NO. 5 and SEQ ID NO. 6. The kit disclosed by the invention has the advantages of being simple and convenient to operate for detection, low in price, and capable of simultaneously quickly detecting all the domestic sheep theileria types at present.

Description

technical field [0001] The present invention relates to a kit for the detection and identification of specific parasites. Specifically, the present invention is a multiplex PCR identification kit that can be used for Theileria ovis, Theileria yoshii, and Theileria ovis. Background technique [0002] Theileria ovis can infect sheep and goats, and has a global distribution. Theileria ovis from different regions may have different biological characteristics and pathogenicity. According to the shape of the worm, the transmission medium, the pathogenicity, the culture characteristics, etc., combined with the differences in the 18S rRNA gene sequence of Theileria ovis, the Theileria ovis is currently divided into six species, namely, Theileria reinhardt, Theileria ovis, and isolated Theileria, Theileria hidden, Theileria lushii, and Theileria yoshii. In my country, the reported pathogens of Theileria in sheep include Theileria yoshii, Theileria rushii and Theileria ovis. The tr...

AI Technical Summary

Problems solved by technology

[0003] At present, the interspecies identification of Theileria mainly relies on the PCR detection of a single insect species. The detection time is long and the steps are complicated, which cannot well meet the rapid detection and identification of field samples.

[0004] At present, there are some methods for identifying Theileria bovine in China, but most of them are identified by single PCR or serological method for Theileria sheep, which takes a long time to identify and the steps are relatively cumbersome.

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