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Construction for number 1 chromosome substitution laboratory mouse strain C57BL/6-Chr1NZW

A technology of chromosome replacement and chromosome, applied in animal husbandry and other fields

Inactive Publication Date: 2013-06-05
上海西普尔-必凯实验动物有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to technical difficulties in the construction of chromosome replacement strains, the inbred strain NZW has not been introduced into the genome of the recipient strain C57BL / 6 mice

Method used

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  • Construction for number 1 chromosome substitution laboratory mouse strain C57BL/6-Chr1NZW
  • Construction for number 1 chromosome substitution laboratory mouse strain C57BL/6-Chr1NZW
  • Construction for number 1 chromosome substitution laboratory mouse strain C57BL/6-Chr1NZW

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0097] Embodiment 1, the construction method of chromosome replacement mouse

[0098] Adult mice of two strains of NZW and C57BL / 6 were taken, and NZW was used as the female parent, and C57BL / 6 was used as the male parent to cross each other to obtain the F1 generation.

[0099] C57BL / 6 was obtained by crossing F1 generation male mice with C57BL / 6 female mice 1 -Chr1 NZW , for backcross generation, in C57BL / 6 1 -Chr1 NZW In male mice, the sex chromosomes and mitochondrial DNA in NZW have been replaced with C57BL / 6; F2 generation male mice were crossed with C57BL / 6 female mice to obtain C57BL / 6 2 -Chr1 NZW , in this way, ten generations of recurrent crosses were obtained, and ten generations of backcrossed mice C57BL / 6 were obtained. 10 -Chr1 NZW , the schematic diagram of the construction process is as follows figure 1 .

Embodiment 2、1

[0100] Example 2, No. 1 chromosome genome identification

[0101] (1) Primary Screening and Identification of Chromosome 1 Genome

[0102] Compare the gene sequences of chromosome 1 of the two strains of NZW and C57BL / 6 (refer to the public information of MGI (http: / / www.informatics.jax.org / ) for the sequence), and select representative and two strains Different SNP sites, these SNP sites are evenly distributed on chromosome 1, and are easy to design and operate. Then design primers for these SNP sites to amplify sequences with characteristic SNP sites, and design the range of PCR amplified fragments between 100 and 400 bp. Primer design is mainly based on primer3 online software (http: / / frodo.wi .mit.edu / primer3 / ) and the Oligo6.0 program (Molecular Biology Insights Inc., USA) were completed. Site-specific probes and general probes were designed for these SNP sites for LDR, and the length of LDR probe ligation products was 80-130 bp.

[0103] The 12 SNP loci of chromosomes...

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Abstract

The invention relates to construction for a number 1 chromosome substitution laboratory mouse strain C57BL / 6-Chr1NZW. According to the construction for the number 1 chromosome substitution laboratory mouse strain C57BL / 6-Chr1NZW, a new chromosome substitution laboratory mouse strain is constructed. A genetic background of the chromosome substitution laboratory mouse strain is based on a C57BL / 6 mouse, while wherein a number 1 chromosome is from an NZW mouse. According to the construction for the number 1 chromosome substitution laboratory mouse strain C57BL / 6-Chr1NZW, the chromosome substitution laboratory mouse strain can be used for research of character differences brought by different number 1 chromosome genomes under the same genome background, and can be a useful tool for research of the chromosome genome functions by adoption of a genetic method.

Description

technical field [0001] The invention belongs to the field of mouse strain construction, in particular relates to the construction of a No. 1 chromosome replacement experimental mouse strain C57BL / 6-Chr1 NZW . Background technique [0002] From a medical point of view, the main challenge in the post-genomic era is to analyze the molecular polymorphisms that lead to the occurrence of common human diseases or complex trait diseases and the differences between individuals. The research on genes related to complex traits is the core task of this period. More than 3,000 QTLs have been mapped so far, but only about 20 genes that regulate complex traits have been clearly identified. In order to meet the challenges of complex trait-related gene research and overcome the current problem of fine mapping of complex trait-related genes, developed countries such as Europe and the United States are exploring and developing new mouse genetic resources. So far, scientists from the United S...

Claims

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Application Information

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IPC IPC(8): A01K67/027
Inventor 赵莹赵丽亚刘磊邢正弘陈国强肖君华
Owner 上海西普尔-必凯实验动物有限公司
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