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Cutting propagation method of strengthening antipollution factor zelkova schneideriana

A cutting propagation, anti-pollution technology, applied in the field of plant propagation

Active Publication Date: 2013-06-05
浙江红叶园艺有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004]At this stage, the research on beech is still limited to the laboratory stage, and there are still many problems that have not been solved, and it has not really been put into factory production, because forest trees The application of tissue culture in actual production is still subject to many restrictions. There are two main reasons
[0005]One is that the growth rate of forest trees is relatively slow, and secondary metabolites accumulate a lot, and browning and vitrification often occur in the tissue culture process;
[0006] Second, the effective rate of plant tissue culture regenerated plants is low, making the propagation technology difficult to meet the needs of actual production. The biological characteristics of zelkova determine its growth rate. Slow, it is difficult to directly obtain explants, and the pollution rate is not easy to control. If seeds are used as explants, there may be certain advantages, but the collection of seeds and the possible problems of germination size and age also bring some problems
[0008] In order to solve the problem of low callus differentiation into adventitious buds, it is urgent to screen and discover more suitable medium and induction methods

Method used

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  • Cutting propagation method of strengthening antipollution factor zelkova schneideriana

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] The young twigs of the zelkova trees are collected in the urban area and other nearby cities affected by haze weather. Observe the trees in good condition and collect the branches that do not affect the growth of the zelkova trees.

[0030] 1) Use 2-3 year old zelkova tree branches as spikes, disinfect the cut with 75% alcohol for 18 seconds, rinse with deionized water, soak with 0.08% nano silver titanium solution for 10 minutes, and rinse with deionized water 3 times, use filter paper to absorb the water for later use;

[0031] 2) Cut the branches treated in step 1) into 1.5cm stems containing at least one axillary bud. The laboratory controlled cultivation environment is normal temperature and pressure, and the air humidity is 90%; the stems are implanted in the culture medium and cultivated 28 In the last 3 days, the clump buds are obtained, and the clump buds are raised for the last 3 days, and the humidity is controlled at 75%. Among them, the formulation of t...

Embodiment 2

[0046] The third-generation seedlings obtained in Example 1 were transplanted into a nutrient bowl with clean water to wash the medium. The nutrient soil in the nutrient bowl was plant ash and humus soil and mixed 1:5, adding a little humus beech leaves as the substrate , Add water to moisturize, control the light intensity to 1500Lx, the daylight is more than 8 hours, control the humidity 75%, after 5 days of treatment, put the nutrient bowl in the greenhouse for normal culture, and check the survival rate of this batch of seedlings after 1 month. Up to 95.6%.

Embodiment 3

[0048] The third-generation seedlings obtained in Example 1 were planted in the open air at the Lin'an Hengfan Hongye Horticulture Planting Base. The sunny plots with more than 8 hours of sunlight were planted by digging holes in the soil, and the ratio of plant ash to humus soil in the pits It is 1:5 mixing, adding a little humus beech leaves as the substrate, and using automatic sprinkler irrigation facilities for irrigation. After one month, the survival rate of the seedlings is 88%.

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Abstract

The invention belongs to the technical field of plant propagation, in particular to a cutting propagation method of strengthening antipollution factor zelkova schneideriana. Branches of the zelkova schneideriana with the tree age of 2 to 3 are utilized as spike strips, cuts are disinfected by alcohol, flushed by deionized water, soaked by a nano-silver titanium solution for 5-10 minutes, flushed by the deionized water again and reserved for use after moisture is sucked. Branches after treatment is cut to be stem sections, the stem sections are planted into a culture medium for culturing for 25 to 30 days, and multiple shoots are obtained. Big and robust shoots are transferred to a root culture media, and culture seedlings are obtained. The culture seedlings are arranged in a greenhouse for 5 to 7 days, the seedlings are cleaned, cultured and transplanted into humus, the culture seedlings with good growth conditions are screened out for secondary-generation culture and continuous optimizing, and seedlings comes out in a third generation. Rooting percentage of the zelkova schneideriana in cutting propagation is improved, rapid expanding propagation of fine breeds of the zelkova schneideriana is promoted, and resistance of the seedlings of the zelkova schneideriana is effectively improved.

Description

technical field [0001] The invention belongs to the technical field of plant propagation, and in particular relates to a cutting propagation method of beech trees with enhanced anti-pollution factors. Background technique [0002] Beech is an arbor tree species belonging to the genus Beech in the family Ulmaceae. It is a national second-class key protected plant and is a precious hard broad-leaved tree species. The beech tree is hard and fragrant, with purple-red heartwood, dense texture, beautiful color, strong pressure resistance, water and humidity resistance, corrosion resistance, and no cracking. It is one of the precious hard broad-leaved timber species in my country. The precious material of furniture is deeply loved by the masses. At present, there are not many large-scale cultivation of beech trees in China, but the market demand for beech trees is very large, so the large-scale cultivation and reproduction of beech trees is imminent, and it also complies with the n...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00A01G1/00
Inventor 余鸿钧丁青松蒋寿松刘霞
Owner 浙江红叶园艺有限公司
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