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Method for rapidly cultivating chlorella

A cultivation method, the technology of chlorella, applied in the biological field, achieves the effect of high efficiency and fast growth rate

Inactive Publication Date: 2013-05-22
YANCHENG INST OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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  • Method for rapidly cultivating chlorella
  • Method for rapidly cultivating chlorella
  • Method for rapidly cultivating chlorella

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Experimental program
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Effect test

Embodiment 1

[0022] 1) Use fresh tap water as a solvent to prepare a standard BG-11 medium for the basal medium of Chlorella; the standard BG-11 medium component (g / L): NaNO 3 , 1.5; K 2 HPO 4 ·3H 2 O, 5.24×10 -2 ;MgSO 4 ·7H 2 O, 7.5×10 -2 ;CaCl 2 , 2.72×10 -2 ; citric acid, 6.562 × 10 -3 ; ferric ammonium citrate, 6.0 x 10 -3 ;EDTANa 2 2H 2 O, 1.1×10 -3 Na 2 CO 3 , 2.0×10 -2 ;H 3 BO 3 , 2.86×10 -3 ;MnCl 2 4H 2 O, 1.86×10 -3 Na 2 MoO 4 2H 2 O, 3.9×10 -4 ; ZnSO 4 ·7H 2 O, 2.2×10 -4 ; CuSO 4 ·5H 2 O, 8.0×10 -5 ; Co(NO 3 ) 2 ·6H 2 O, 5.0×10 -5 .

[0023] 2) Add a certain amount of glucose and NaHCO to the standard medium 3 , so that the final concentrations were 0.5g / L and 0.2g / L.

[0024] 3) Put the above-mentioned medium into a culture container, inoculate 10% chlorella liquid, and place the algae liquid in an environment with light intensity of 3500 Lx, light-to-dark ratio of 12h:12h, and temperature of 25°C for cultivation.

[0025] The OD value of th...

Embodiment 2

[0027] 1) Use fresh tap water as a solvent to prepare a standard BG-11 medium for the basal medium of Chlorella; the standard BG-11 medium component (g / L): NaNO 3 , 1.5; K 2 HPO 4 ·3H 2 O, 5.24×10 -2 ;MgSO 4 ·7H 2 O, 7.5×10 -2 ;CaCl 2 , 2.72×10 -2 ; citric acid, 6.562 × 10 -3 ; ferric ammonium citrate, 6.0 x 10 -3 ;EDTANa 2 2H 2 O, 1.1×10 -3 Na 2 CO 3 , 2.0×10 -2 ;H 3 BO 3 , 2.86×10 -3 ;MnCl 2 4H 2 O, 1.86×10 -3 Na 2 MoO 4 2H 2 O, 3.9×10 -4 ; ZnSO 4 ·7H 2 O, 2.2×10 -4 ; CuSO 4 ·5H 2 O, 8.0×10 -5 ; Co(NO 3 ) 2 ·6H 2 O, 5.0×10 -5 .

[0028] 2) Add a certain amount of fructose and NaHCO to the standard medium 3 , so that the final concentrations were 0.5g / L and 0.2g / L.

[0029] 3) Put the above culture medium into a culture container, inoculate with 10% chlorella liquid, seal the seal, and place the algae liquid in an environment with light intensity of 3500Lx, light-to-dark ratio of 12h:12h, and temperature of 25°C for cultivation.

[0030] ...

Embodiment 3

[0032] 1) Use fresh tap water as a solvent to prepare a standard BG-11 medium for the basal medium of Chlorella; the standard BG-11 medium component (g / L): NaNO 3 , 1.5; K 2 HPO 4 ·3H 2 O, 5.24×10 -2 ;MgSO 4 ·7H 2 O, 7.5×10 -2 ;CaCl 2 , 2.72×10 -2 ; citric acid, 6.562 × 10 -3 ; ferric ammonium citrate, 6.0 x 10 -3 ;EDTANa 2 2H 2 O, 1.1×10 -3 Na 2 CO 3 , 2.0×10 -2 ;H 3 BO 3 , 2.86×10 -3 ;MnCl 2 4H 2 O, 1.86×10 -3 Na 2 MoO 4 2H 2 O, 3.9×10 -4 ; ZnSO 4 ·7H 2 O, 2.2×10 -4 ; CuSO 4 ·5H 2 O, 8.0×10 -5 ; Co(NO 3 ) 2 ·6H 2 O, 5.0×10 -5 .

[0033] 2) Add a certain amount of sucrose and NaHCO to the standard medium 3 , so that the final concentrations were 0.5g / L and 0.2g / L.

[0034] 3) Put the above culture medium into a culture container, inoculate with 10% chlorella liquid, seal the seal, and place the algae liquid in an environment with light intensity of 3500Lx, light-to-dark ratio of 12h:12h, and temperature of 25°C for cultivation.

[0035] T...

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Abstract

The invention relates to a method for rapidly cultivating chlorella and belongs to the technical field of biology. The cultivation method comprises the following steps: preparing a standard BG-11 culture medium to serve as a basal culture medium of chlorella by taking fresh running water as a solvent; adding a certain amount of organic carbon source and NaHCO3 into the standard culture medium, so that the final concentration is respectively 0.5-1.0g / L and 0.2-0.5g / L; putting the culture medium into a culture vessel, inoculating 10-20 percent of chlorella solution, sealing the opening, and culturing the chlorella solution in an environment with the illumination intensity of 3000-4000Lx, the light-dark ratio of 12h:12h and the temperature of 22-28 DEG C. According to the method, the cell density of the chlorella can reach a high level in a short cultivating cycle and is increased by about six times compared with that cultivated by using the basal culture medium. Meanwhile, the method has the advantages that the process is easy to operate, the culture medium is not required to be sterilized, the pH value of the culture medium is not required to be regulated, and the method can be widely applied to cultivating chlorella in industrial production.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to a method for quickly cultivating chlorella under mixed culture conditions. Background technique [0002] Chlorella is a single-cell green algae of the genus Chlorella in the phylum Chlorella. It is widely distributed, has strong environmental adaptability, and a short growth cycle. It is a new type of low-carbon environmental protection resource. Chlorella is rich in protein, polysaccharides, lipids, leaf greens, vitamins and some biologically active substances. It is widely used in health food, feed, food additives, fine chemicals and pharmaceutical preparation raw materials, and has important development potential. Therefore, it is particularly important to obtain a large number of high-quality chlorella cells quickly, easily and cheaply. [0003] So far, there are many methods for rapidly cultivating Chlorella reported, but there are few methods suitable for industrialized large-sc...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/12C12R1/89
Inventor 许伟颜秀花吴乔林齐志涛邵荣
Owner YANCHENG INST OF TECH
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