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Preparation method of natural preservative regarding Chinese pear-leaved crabapple leaves as raw material and application thereof

A technology of natural preservatives and main raw materials, applied in applications, preservation of meat/fish with chemicals, food science, etc., can solve the problems of sodium benzoate dosage and use range limitations, and achieve low production cost, good inhibitory effect, and enhanced The effect of antibacterial effect

Inactive Publication Date: 2013-05-15
ZHEJIANG OCEAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The use of chemical preservatives in countries around the world is becoming more and more strict. Developed countries such as Japan have completely banned the application of sodium benzoate in food. Although my country still allows the use of sodium benzoate in food, it has also made the dosage and scope of sodium benzoate. strict restrictions on

Method used

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  • Preparation method of natural preservative regarding Chinese pear-leaved crabapple leaves as raw material and application thereof
  • Preparation method of natural preservative regarding Chinese pear-leaved crabapple leaves as raw material and application thereof
  • Preparation method of natural preservative regarding Chinese pear-leaved crabapple leaves as raw material and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0013] Weigh 1 portion of Linopodium leaves, clean them, put them in a drying oven at 40°C, and dry them to a moisture content of 15%, crush them and pass through a 60-mesh sieve; add 20 times the mass of Linopodium leaves powder with a percentage concentration of 60% Ethanol, heated and refluxed twice at 50°C for 5 hours each time, collected the reflux liquid twice, combined, filtered, transferred to a rotary evaporator and concentrated to 0.4g original drug / mL, and the concentrated solution was filtered through a microporous membrane or centrifuged ( 10000r / min, 10min) upper LSA-10 macroporous resin column, the mass ratio of Linopodium leaf powder and macroporous resin is 1: 1, the ratio of resin column diameter to height is 1: 4, upper column flow rate 1BV / h; Elute 5BV / h, the elution flow rate is 1BV / h, discard the water eluent; then elute with 60% ethanol, the elution flow rate is 1BV / h, collect the ethanol eluate, and use vacuum drying to remove ethanol. Obtain solid leaf...

Embodiment 2

[0015] Weigh 1 portion of Linopodium leaves, clean them, put them in a drying oven at 40°C, and dry them to a moisture content of 16%, crush them and pass through a 60-mesh sieve; add 25 times the mass of ethanol with a concentration of 75% to the powder of Linopodium leaves , refluxed 3 times at 50°C, 3 hours each time, collected reflux liquid for 3 times, combined, filtered, transferred to a rotary evaporator and concentrated to 0.6g original drug / mL, and the concentrated solution was filtered through a microporous membrane or centrifuged (10000r / min, 10min) on the LSA-10 macroporous resin column, the mass ratio of Linopodium leaf powder to macroporous resin is 1:2, the ratio of resin column diameter to height is 1:6, and the column loading speed is 3BV / h; water elution 8BV, the elution rate is 2BV, and the water eluent is discarded; then eluted with ethanol with a percentage concentration of 75%, the elution flow rate is 2BV / h, the ethanol eluate is collected, and the ethan...

Embodiment 3

[0017] Weigh 1 portion of Linopodium leaves, clean them, put them in a drying oven at 40°C, and dry them to a moisture content of 18%, crush them and pass through a 60-mesh sieve; add 30 times the mass of ethanol with a concentration of 95% to the powder of Linopodium leaves , refluxed 4 times at 50°C, 2 hours each time, collected the reflux liquid for 4 times, combined, filtered, transferred to a rotary evaporator and concentrated to 1.0g of the original drug / mL, and the concentrated solution was filtered through a microporous membrane or centrifuged (10000r / min, 10min) on the LSA-10 macroporous resin column, the mass ratio of Linopodium leaf powder to macroporous resin is 1:4, the ratio of resin column diameter to height is 1:8, and the column loading speed is 5BV / h; water elution 10BV, the elution rate is 3BV, and the water eluent is discarded; and then eluted with ethanol with a percentage concentration of 95%, the elution flow rate is 3BV / h, the ethanol eluate is collecte...

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Abstract

The invention discloses a preparation method of a natural preservative regarding Chinese pear-leaved crabapple leaves as a raw material and an application thereof. The preparation method comprises the following steps of: cleaning, drying and smashing the Chinese pear-leaved crabapple leaves, adding ethanol, heating, refluxing, extracting, filtering and combining the extracting solution, concentrating the treated extracting solution, filtering the extracting solution by microporous membrane, or centrifuging, and introducing the filtered extracting solution to pass through macroporous resin; and firstly washing the macroporous resin with water, abandoning the water eluant, eluting by using the ethanol, collecting the ethanol eluant, concentrating and removing the ethanol eluant to obtain a Chinese pear-leaved crabapple leaf extract, and smashing the extract so as to obtain the natural preservative. The raw material used in the method belongs to the leaves of a natural plant, so that the raw material is a renewable resource and has big resource preservation amount and low production cost. The natural preservative prepared by the invention has the good inhibiting effect on a plurality of microorganisms including gram-positive bacteria and gram-negative bacteria, is capable of enhancing the antibacterial effect by being compatible with other auxiliary materials such as citric acid and sorbic acid, and can be applied to anticorrosion on aquatic and animal products, and the like.

Description

technical field [0001] The invention relates to the field of preservatives, in particular to a preparation method and application of a natural preservative using Linopodium leaves as the main raw material. Background technique [0002] Food preservation is an old proposition. Since the emergence of human beings, they have consciously or unconsciously invented food preservation methods such as salting and smoking. So far, food preservation is still one of the main research contents of food scientists. It has been proven that there are many factors affecting food spoilage, such as microorganisms, light, oxygen, moisture, etc. Among them, the most important factor causing food spoilage is microorganisms. Most food spoilage is caused by microorganisms. Since then, preservatives have been born due to luck. The main means to maintain and extend the shelf life of food in the food industry is also to use preservatives. Preservatives can be divided into chemical preservatives (a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23L3/3472A23B4/20
Inventor 霍健聪邓尚贵
Owner ZHEJIANG OCEAN UNIV
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