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Method for auxiliary identification of corn haploid induction line

A haploid inducible line, corn technology, applied in the directions of botanical equipment and methods, application, plant genetic improvement, etc., can solve the problems of inducible line non-scattering powder, economic loss, lack of fruit, etc., and achieves emphasis on screening and accelerated breeding. process, the effect of increasing the efficiency of breeding

Active Publication Date: 2013-05-08
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the complexity of breeding for induced traits, it is difficult to select the induction rate while taking into account agronomic traits, etc., resulting in poor adaptability of the induced line itself
The climatic conditions in various ecological regions in my country are complex, and it is difficult for the existing induction system to be widely used all over the country. In addition, under unsuitable climatic conditions, the induction system may not loose powder, not firm, etc., causing huge economic losses

Method used

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  • Method for auxiliary identification of corn haploid induction line
  • Method for auxiliary identification of corn haploid induction line
  • Method for auxiliary identification of corn haploid induction line

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0062] Example 1. In different inbred lines, the rate of haploid induction is significantly related to the fruitiness, endosperm abortion and embryo abortion of selfed ears

[0063] Maize inbred lines CAU5, CAUHOI, CAU079, UH400, N1680, B73, Mo17, BY815, GY923, GY302, GY220, P138, Zong 3, Zong 31, 178, Huang C, 1145. Among them, CAU5, CAUHOI, CAU079 and UH400 are haploid induction lines, and the rest are conventional inbred lines.

[0064] According to the above definitions, the self-fertilization rate, endosperm abortion rate, embryo abortion rate and haploid induction rate of the above 17 materials were analyzed, and the haploid induction rate of the 4 induced lines was carried out according to the above definition method. Haploid induction rates were not calculated for the remaining 13 conventional inbred lines. Studies have shown that maize is also diploid (2n=20), and the natural occurrence frequency of haploid (2n=10) is very low, only 0.1% (Chase SS. Monoploid frequenc...

Embodiment 2

[0074] Example 2, Application of Grain Firmness, Endosperm Abortion, and Embryo Abortion in the Breeding of Haploid Inducer Lines

[0075] 1. Materials

[0076] High-oil corn inbred line N1680: N1680 is a high-oil inbred line with an average oil content of 8%, no haploid induction rate, and no R-nj marker;

[0077] High-frequency haploid induction line CAU5: CAU5 is a high-frequency haploid induction line with an average induction rate of 10% and R-nj marker;

[0078] The species tested for induction rate was Zhengdan 958.

[0079] Two, the inventive method

[0080] The following experiments were all carried out at the Beijing Shangzhuang Experimental Station of China Agricultural University. Unless otherwise specified, the row length was 3m, the row spacing was 0.6m, and the plant spacing was 0.25m. Single seed was sown, with 13 seeds per row.

[0081] (1) Hybrid 1:

[0082] The induced line CAU5 is an early-maturing material, and it takes 65 days from sowing to loose pow...

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Abstract

The present invention discloses a method for auxiliary identification of a corn haploid induction line. The method comprises the following steps: detecting a seed setting rate, an endosperm abortion rate and an embryo abortion rate of corn requiring detection, wherein the corn candidate requiring detection is determined as the corn haploid induction line if the seed setting rate, the endosperm abortion rate and the embryo abortion rate of the corn requiring detection meet the following conditions such as 1), 2) or 3). The method is particularly suitable for cultivation of a haploid induction line capable of carrying out haploid induction in a target area ??environment, wherein corn suitable for growing in the target area is adopted as a female parent, a known haploid induction line is adopted as a male parent, and operations are performed according to the method, such that the haploid induction line having a haploid induction character of the male parent and a target area ??environment adaptability character of the female parent can be obtained.

Description

technical field [0001] The invention relates to a method for assisting the identification of maize haploid induction lines. Background technique [0002] The main way of producing maize haploid is the induction line hybridization induction pathway to produce maternal (female) haploid, referred to as parthenogenetic haploid. Parthenogenetic haploids can be produced through biological induction and abiotic induction (such as chemical induction), but the problem of abiotic induction is as difficult as that of anther tissue culture, and it is difficult to apply it in breeding. Biological induction is the use of biological induction materials to make the induced object produce haploid. Coe (Coe E H.A line of maize with high haploid frequency [J]. Am Nat, 1959, 93: 381-382) discovered the maize parthenogenic induction line Stock6 in 1959. Later, it was continuously modified on its basis, such as the introduction of the Navajo (ACR-nj) genetic marker gene, and the haploid was ide...

Claims

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Application Information

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IPC IPC(8): A01H1/04A01H1/02
Inventor 陈绍江徐小炜黎亮董昕
Owner CHINA AGRI UNIV
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