Automatic culture device

Abstract

In order to uniformly sow cells on the culture surface of a cartridge-type closed culture container and remove bubbles contaminating a liquid culture medium in the course of automatic culture, an automatic culture device is provided with a movement-controlling mechanism section for solving the non-uniformity in cell distribution by, after filling up a culture space in the cartridge-type closed culture container, said cartridge-type closed culture container being in an upright position, with a cell suspension, turning the cartridge-type closed culture container into a horizontal position, and then repeatedly supplying the cell suspension and sucking the same multiple times to thereby create a mixing flow in the cell suspension. In this process, the liquid in channels is efficiently sent by applying a reduced pressure and an elevated pressure respectively to a channel on one side and a channel on the opposite side, using two syringes and check valves connected to the channels, to thereby load forces to the liquid from both sides. The liquid can be sent without any cell loss by conducting the same operations of the movement-controlling mechanism section in a tank and in a cell bag.

Description

technical field [0001] The present invention relates to an automatic culture device for culturing cells or tissues through automatic operation using a box-type closed culture container, for example, an automatic culture device capable of producing regenerative tissues that can be used in regenerative medicine. Background technique [0002] Manufacture of regenerative tissues used for transplantation or the like as regenerative medical treatment is based on GMP (Good Manufacturing Practice) which is a standard for manufacturing management and quality control of drugs and the like. Usually, regenerated tissue is manufactured by a manufacturer with specialized cell culture technology in a CPC (Cell Processing Center; cell processing facility) that provides a clean manufacturing environment in accordance with SOP (Standard Operational Procedure; standard operating manual). In Japan, in terms of GMP, laws and regulations prescribed by the Ministry of Health, Labor and Welfare, su...

AI Technical Summary

Problems solved by technology

Culture technology using closed culture containers and automatic culture equipment has not yet been fully established, but several reports have been made (Patent Documents 1 and 2)

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