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Application of SHSP60 for preparing diagnostic reagent for monitoring comprehensive hepatic pathology injury degree of schistosomiasis patient

A technology for schistosomiasis and disease patients, applied in the field of immunology, to achieve the effect of broad application prospects

Inactive Publication Date: 2014-08-13
NANJING MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there has not been any report so far that someone has used schistosomiasis HSP for early estimation and process monitoring of liver immunopathological damage in patients with schistosomiasis to guide clinical intervention. , Doumbo et al.1990)

Method used

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  • Application of SHSP60 for preparing diagnostic reagent for monitoring comprehensive hepatic pathology injury degree of schistosomiasis patient
  • Application of SHSP60 for preparing diagnostic reagent for monitoring comprehensive hepatic pathology injury degree of schistosomiasis patient
  • Application of SHSP60 for preparing diagnostic reagent for monitoring comprehensive hepatic pathology injury degree of schistosomiasis patient

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Example 1: In order to study whether the serum SjHSP60-IgG and its subtype IgG1 antibody titers are positively correlated with the average area of ​​single egg granuloma and fibrosis degree of the corresponding liver in the rabbit model infected with Schistosoma japonicum.

[0037] 1. Put the snails in clean dechlorinated water at 25°C under light conditions to escape the cercariae. The cercariae were picked with an inoculation loop, moved to a cover glass (12mm×12mm), and counted under a dissecting microscope. Each group of mice was infected with 12, 20, 40 cercariae, respectively. The mice and rabbits were bound and fixed, and their abdomens were shaved. The skin was moistened with dechlorinated water, and the coverslip with cercariae was covered on it, and kept for 20 minutes. Mice and rabbits were sacrificed at 8 and 23 weeks after infection, respectively (Xu, Wen et al. 2010).

[0038] 2. Take the right anterior lobe liver. After being fixed with 10% neutral for...

Embodiment 2

[0041] Example 2: In order to study whether the serum SjHSP60-IgG antibody titer is positively correlated with the average area of ​​single egg granuloma and the degree of fibrosis of the corresponding liver in the rabbit model infected with Schistosoma japonicum.

[0042] 1. According to the method in Example 1, each group of rabbits was infected with 20, 40 and 800 cercariae respectively. The level of SjHSP60 IgG antibody in serum was detected by ELISA.

[0043] 2. Then take the right anterior lobe liver. After being fixed with 10% neutral formaldehyde, routinely dehydrated and embedded in paraffin, the sections were stained with H.E. The granuloma area of ​​a single egg was observed under a Zeiss microscope, and 30 areas were observed for each egg, and the average value was calculated. To evaluate the degree of liver fibrosis in infected rabbits, collagen fibers I, III and α-SMA were sectioned in paraffin. Then, an experienced pathologist evaluated the protein expression ...

experiment example 3

[0047] Experimental Example 3: In order to study whether the serum SjHSP60 antibody titer is also positively correlated with the degree of liver fibrosis in the schistosome infection mouse model and other factors that promote liver fibrosis.

[0048] 1. According to the method in Example 1 (1), the mice were infected with Schistosoma japonicum, and the mice were treated with carbon tetrachloride and PBS in different groups at the same time, and the levels of SjHSP60 IgG and its subtype IgG1 antibodies in the serum of the mice in each group were detected.

[0049] 2. Then take the right anterior lobe liver. After being fixed with 10% neutral formaldehyde, routinely dehydrated and embedded in paraffin, the sections were stained with H.E. The granuloma area of ​​a single egg was observed under a Zeiss microscope, and 30 areas were observed for each egg, and the average value was calculated.

[0050] 3. SjHSP60 and MouseHSP60 were used to coat the plate with OVA protein, and ELIS...

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Abstract

The invention belongs to the field of immunology, and particularly relates to an application of proteantigen molecules from schistosomes such as Japanese schistosome shock proteins 60KDa (SjHSP60), which is the application of Japanese schistosome shock proteins 60KDa for preparing a diagnostic reagent for monitoring comprehensive hepatic pathology injury degree of a schistosomiasis patient. The SjHSP60IgG in blood serum of a Japanese schistosome infected host and subtype IgG1 antibody level thereof are positively correlated to liver granuloma and fibrosis degree, which promotes that the SjHSP60IgG in blood serum and the subtype IgG1 antibody level thereof can be used as an index of evaluating the pathological degree of the liver of the schistosomiasis patient and has potential clinical application value. In the future, the novel diagnostic reagent may be used for monitoring the pathological degree of the liver of the schistosomiasis patient.

Description

technical field [0001] The invention belongs to the field of immunology, and in particular relates to schistosoma japonicum heat shock protein 60KDa (SjHSP60) IgG and its subtype IgG1 antibody in serum of a host infected with schistosoma japonicum and its application. Background technique [0002] Schistosomiasis is one of the most serious parasitic diseases in my country and even in the world. Globally, about 600 million people are now threatened and 200 million people are infected, of which 20 million people have severe clinical symptoms and are accompanied by varying degrees of labor loss. In my country, by the end of 2008, schistosomiasis was still prevalent in 105 counties in seven provinces, municipalities, and autonomous regions, and about 460,000 cases of schistosomiasis were reported, nearly 100 million people were threatened by infection, and livestock infection in epidemic areas was more common (Gryseels, Polman et al. 2006; Hotez, Brindley et al. 2008). The etio...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/68
Inventor 苏川陈晓军周莎
Owner NANJING MEDICAL UNIV
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