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Method for preparing rehydration bacterial cellulose membrane by utilizing vegetable protein and hydrolysate thereof

A technology of bacterial cellulose membrane and bacterial cellulose is applied in the field of preparing rehydration bacterial cellulose membrane, which can solve the problem of not finding rehydration bacterial cellulose membrane, and achieve the effect of excellent rehydration performance and high affinity

Inactive Publication Date: 2013-04-24
天津市工业微生物研究所有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] After searching, it has not been found to use plant protein and its hydrolyzate to prepare rehydrated bacterial cellulose membrane, so as to ensure that the dried bacterial cellulose membrane can still restore the original gel state

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] (1) Soak 15g of the cellulose gel film that has been removed from the bacteria and residual culture medium in the dissolved 10% soybean protein hydrolyzate, soak the front side for 20 minutes, and the reverse side for 20 minutes. After soaking, the bacterial cellulose wet film weighs 22.6039 g.

[0022] (2) Dry the soaked cellulose film in an oven at 105°C for 4 hours to obtain a rehydrated bacterial cellulose film with a dry weight of 1.2518g. Put the cellulose film back into distilled water for 1 hour to make it swell. After rehydration, the weight of the cellulose wet film is 12.33g. After rehydration, the water content of the cellulose film reaches 89.85%. Compared with the wet film before drying, the rehydration rate It is 51.88%.

[0023] The formula for calculating the rehydration rate is as follows:

[0024] Rehydration rate = (wet weight after rehydration - dry weight) / (wet weight of original film - dry weight)

[0025] Blank test: Soak 15g of the cellulos...

Embodiment 2

[0027] (1) Soak 15g of the cellulose gel film that has removed bacteria and residual culture medium in 0.5% wheat protein solution that has been dissolved, soak the front side for 40 minutes, and the reverse side for 40 minutes. The wet weight of the bacterial cellulose film after soaking is 20.2089 g;

[0028] (2) Dry the soaked cellulose film in an oven at 105°C for 5 hours to obtain a rehydratable bacterial cellulose film with a dry weight of 0.437 g. Put the cellulose film back into distilled water for 1 hour to make it swell, and then rehydrate it. The weight of the cellulose wet film after watering was 5.91g, and the water content of the cellulose film after rehydration reached 92.61%, which was 27.68% compared with the wet film before drying.

Embodiment 3

[0030] (1) Soak 15g of the cellulose gel film that has been removed from the bacteria and residual culture medium in the 10% soybean peptide solution that has been dissolved, soak the front side for 30 minutes, and the reverse side for 30 minutes. After soaking, the bacterial cellulose wet film weighs 18.1567g ;

[0031] (2) Freeze-dry the soaked cellulose membrane to obtain a rehydratable bacterial cellulose membrane with a dry weight of 1.2068g. Put the cellulose membrane back into distilled water for 1 hour to make it swell. After rehydration, the cellulose wet membrane The weight is 12.253g, and the moisture content of the cellulose membrane after rehydration reaches 90.15%, which is 65.17% compared with the wet membrane before drying.

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Abstract

The invention relates to a method for preparing a rehydration bacterial cellulose membrane by utilizing a vegetable protein and hydrolysate of the vegetable protein. The method comprises the following steps of: (1) soaking a bacterial cellulose gel membrane of which a thalli and a residual culture medium are removed into 0.5 percent to 10 percent of vegetable protein liquid or vegetable protein hydrolysate for 10 to 300min, so that protein molecules, peptide molecules or free amino acid are / is diffused to enter the inside of a bacterial cellulose fiber network and be linked to the surface of the bacterial cellulose fiber network; and (2) carrying out oven drying or freeze drying on the processed bacterial cellulose membrane to obtain the rehydration bacterial cellulose membrane. The rehydration bacterial cellulose membrane prepared by the method can effectively improve rehydration capacity and the water holding characteristic of the dried bacterial cellulose membrane; and meanwhile, the rehydration bacterial cellulose membrane comprises the natural plant active protein, and thus, the rehydration bacterial cellulose membrane has various physiological functions of nutrition, moisturizing, aging prevention, regeneration promotion and the like when being applied in the fields of cosmetics and medicine.

Description

technical field [0001] The invention relates to bacterial cellulose membranes of biological materials, in particular to a method for preparing rehydrating bacterial cellulose membranes by using plant proteins and hydrolyzates thereof. Background technique [0002] In recent years, bacterial cellulose (BC) has attracted widespread attention due to its unique properties, especially in the fields of cosmetics and biomedicine. Gluconacetobacter xylinus secretes microfibrils from pores in its own cell wall that aggregate to form cellulose ribbons along one side of the cell. The gel pad-like substance formed on the surface of the culture solution during static fermentation is formed by the interweaving of these fiber ribbons. Bacterial cellulose has high water absorption capacity, can absorb more than 100 times its own weight in water, and is stronger than plant-derived cellulose. The high water-holding property of bacterial cellulose is one of its extremely important properties...

Claims

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Application Information

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IPC IPC(8): C08J7/12C08L1/02A61K8/73A61Q19/00A61Q19/08A61L15/28A61L15/42
Inventor 赵琼温冰徐勇虎王祥河许勤虎韩慧国华范婷墨玉欣
Owner 天津市工业微生物研究所有限公司
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