Horseradish plantlet culture method

A cultivation method and technology for seedlings, applied in the field of wasabi seedling cultivation, can solve the problems of slow reproduction speed, low seedling reproduction coefficient, difficulty in producing a large number of high-quality healthy seedlings, etc., and achieve low utilization rate, high utilization rate, and increase root length. Effect

Inactive Publication Date: 2013-04-24
四川华阳蓝公府实业有限公司
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AI Technical Summary

Problems solved by technology

[0005] It can be seen from the above that the traditional seedling breeding method leads to low seedling reproduction coefficient and slow propagation speed, making it difficult to produce a large number of high-quality healthy seedlings

Method used

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  • Horseradish plantlet culture method

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Embodiment

[0028] (A) Rooting cultivation: Transfer the shoot apical meristems cultivated in the first generation of sterilization and detoxification to 1 / 2MS medium without hormones for growth, and add rooting meristem at a concentration of 4mg / L to 1 / 2MS medium agent, the rooting agent is NAA, keep the temperature at 18-20°C, and light at 2500-3000LUX;

[0029] (B1) Keep the temperature in the greenhouse at 8-20°C, move the rooted bottle seedlings into the greenhouse, open the bottle cap and spray to moisturize for two days;

[0030] (B2) Take out the seedlings and wash off the residual medium on the roots;

[0031] (B3) Use vermiculite, perlite, pastoral soil, and humus to form a matrix in the plug tray and water it thoroughly;

[0032] (B4) Insert the seedlings obtained in step (B2) into the plug tray of step (B3), and pour 1 / 10MS medium solution once a week, and keep the humidity;

[0033] (C) Field planting: Step (B) 75-85 days after transplanting and hardening the seedlings, tra...

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Abstract

The invention discloses a horseradish plantlet culture method which comprises the steps of (A) rootage cultivation: transferring sterilized and detoxified stem tip meristem after primary culture into a 1 / 2 MS culture medium which is free from hormone for growing; (B) transplanting and hardening plantlet: after keeping the step (A) for 28-32d, transplanting into a potted tray; and (C) planting in field: after transplanting and hardening the plantlet in the step (B) for 75-85d, transplanting the product horseradish plantlet into a field. According to the horseradish plantlet culture method disclosed by the invention, a rootage agent is added into the culture medium for the propagation culture to replace cytokinin, so that the problems of comparatively slow rootage and slender root are solved, and a purpose of rapidly lengthening the root of a single seedling is achieved in a short period; meanwhile, the rootage agent is farther lower than the cytokinin in price, and is lower in dosage and higher in utilization rate, and in comparison with the auxin culture way which is comparatively serious in waste of the auxin and low in utilization rate, the culture method greatly reduces the cost.

Description

technical field [0001] The invention relates to a method for cultivating seedlings in the field of agriculture, in particular to a method for cultivating wasabi seedlings. Background technique [0002] In the development of wasabi industry in our country, ink disease (also known as black heart disease) is the most important disease of wasabi planting, which can cause complete failure of wasabi planting in severe cases. Existing studies have found that squirrel disease is a specific disease of wasabi, which does not cause harm to other crops and does not cause adverse effects on human health. Ink disease is caused by the fungus Phoma wasabiae Yokogi invading wasabi plants and infecting wasabi plants mainly through vascular bundle conduction, which seriously affects the quality and yield of wasabi, and even leads to the failure of wasabi harvest. Ink disease can damage the rhizomes, fibrous roots, leaves, and petioles of wasabi. After many years of research in Japan, Taiwan,...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 方季虎
Owner 四川华阳蓝公府实业有限公司
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