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Method for promoting growth of Nannochlorisoculata and increasing contents of chlorophyll and mycoprotein of Nannochlorisoculata

A protein content, chlorella technology, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc.

Active Publication Date: 2014-08-20
NANJING NORMAL UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] Existing studies have shown that by controlling the pyrolysis conditions, cellulosic substances can be effectively converted into a pyrolyzed solution containing a high concentration of endo-ether sugars (1,6-anhydro-βD-glucopyranose), but nature can utilize endo-ether sugar microbes are few

Method used

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  • Method for promoting growth of Nannochlorisoculata and increasing contents of chlorophyll and mycoprotein of Nannochlorisoculata
  • Method for promoting growth of Nannochlorisoculata and increasing contents of chlorophyll and mycoprotein of Nannochlorisoculata
  • Method for promoting growth of Nannochlorisoculata and increasing contents of chlorophyll and mycoprotein of Nannochlorisoculata

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Experimental program
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Effect test

Embodiment 1

[0024] Add 10mM inner ether sugar to 50ml of algae liquid, and another part is used as control (without inner ether sugar), culture at 25°C and 4000Lux light intensity for 5 days (shake well every day), use hemocytometer and optical density Compared with the blank control, the yield increase rate was 92%, the total chlorophyll content increased by 82%, and the bacterial protein increased by 76%. life see figure 1 As shown, the effect on chlorophyll content is shown in image 3 shown).

Embodiment 2

[0026] Add 25mM inner ether sugar to 50ml of algae liquid, and another part is used as control (without inner ether sugar), culture at 25°C, 4000Lux light intensity for 5 days (shake well every day), use hemocytometer and optical density Compared with the control, the yield increased by 194%, the total chlorophyll content increased by 156%, and the bacterial protein increased by 122%.

Embodiment 3

[0028]Add 50mM inner ether sugar to 50ml of algae liquid, and another part is used as control (without inner ether sugar), culture at 25°C, 4000Lux light intensity for 5 days (shake well every day), use hemocytometer and optical density Compared with the control, the yield increased by 155%, the total chlorophyll content increased by 121%, and the bacterial protein increased by 99%.

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Abstract

The invention relates to a method for promoting growth of Nannochlorisoculata and increasing contents of chlorophyll and single-cell protein of the Nannochlorisoculata. The method comprises the step that levoglucosan from biomass pyrolysis or cellulose pyrolysis liquid subjected to detoxification treatment is added to a Nannochlorisoculata culture solution, wherein the additive amount of levoglucosan is 5-50mM, and the additive amount of the cellulose pyrolysis liquid is 0.25-2% by weight. By taking a cellulosic biomass high-temperature pyrolysis product, namely levoglucosan or the pyrolysis liquid as an accelerant, a result shows that levoglucosan or the pyrolysis liquid can promote the growth of the Nannochlorisoculata obviously; a promoting rate reaches up to 194%; and the contents of chlorophyll and mycoprotein can be increased significantly, so that a use value of a cellulosic biomass can be increased.

Description

technical field [0001] The invention relates to a method for promoting the growth of chlorella and increasing the amount of chlorophyll and bacterial protein by using cheap cellulose biomass. Background technique [0002] Chlorella belongs to Chlorophyta, is a common single-celled algae, round or oval in shape, with a wide variety, distributed in fresh water and sea water, and can grow well on artificial medium, is the first Artificially cultivated microalgae (Chen Ying, Li Wenbin, Sun Yongru. Current status and prospects of Chlorella biotechnology research and application [J]. Bioengineering Progress, 1998,18(6):12-16). Chlorella is a good material for biotechnology research because of its wide distribution, fast growth rate and easy cultivation. As an additive, chlorella can be widely used in the food industry, and can be used to produce chlorophyll, proline, and oil (Li Zhiyong, Guo Siyuan, Li Lin. Development and utilization of microalgae health food[J]. Food Research a...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/12C12R1/89
Inventor 陈育如孙欢赵乙萱刘军利卫民
Owner NANJING NORMAL UNIVERSITY
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