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Microbial preparation for inhibiting root-knot nematode and preparation method and application thereof

A technology of microbial preparations and root-knot nematodes, applied in botany equipment and methods, biocides, nematicides, etc., can solve problems such as high cost of use, poor environmental compatibility, and damage to soil structure, and achieve long storage time Effect

Active Publication Date: 2014-11-05
河北衡晓生物科技有限公司
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  • Abstract
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Problems solved by technology

Traditional chemical nematicides are not only expensive to use, but also have poor environmental compatibility, which destroys the structure of the soil

Method used

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  • Microbial preparation for inhibiting root-knot nematode and preparation method and application thereof

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Experimental program
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Effect test

Embodiment

[0031] The original strains of Streptomyces avermitilis, Paecilomyces lilacinus and Verticillium chlamydomus were plated with fungal culture medium, and the original strains of Bacillus sphaericus were activated and cultured in the laboratory for 1 day using the bacterial culture medium slope. After activation, Each strain was cultured in a shake flask with the same composition as the respective activation medium for 2 days, and then the strains cultured in the shake flask were respectively placed in a 500L fermenter with the same 10% concentration of mashed potatoes and The culture medium of 3% soybean meal powder was cultivated for 27 hours. Finally, the 500L fermenter cultured Streptomyces avermitilis, Paecilomyces lilacinus, Verticillium chlamydosporium and Bacillus sphaericus, and the sterilized glycerin were 30%, 15% %, 25%, 10% and 20% are mixed.

experiment example

[0032] Experimental example: In mid-March 2012, the experimental work of the microbial preparation for inhibiting root knot nematodes was carried out in Daping Township, Tongcheng County, Hubei Province and Anjiang Town, Huaihua City, Hunan Province. The demonstration area is 130 mu. During the demonstration, the soil conditions, water and fertilizer and various management levels are consistent. The demonstration fields are all greenhouses where celery has been grown for many years, and the root-knot nematode disease is serious. The plot is carefully cultivated, carefully managed and protected every year, and a large amount of money has been invested in the purchase of chemical fertilizers and pesticides to increase the fertility of the land and to eliminate diseases and insect pests. However, with the increase of planting time, root-knot nematodes have become more and more severe. The area is reduced by more than 40%. After using this invention this year, the vegetable field ...

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Abstract

The invention belongs to the field of biological prevention and control on crop insect damage and provides a microbial preparation for inhibiting root-knot nematode. The preparation is formed by mixing bacterial liquids of streptomyces avermitilis, paecilomyces lilacinus, verticillium chlamydosporium and Bacillus sphaericus and glycerol. The invention also provides a production method and an application effect of the microbial preparation for inhibiting the root-knot nematode. The microbial preparation comprises viable bacteria of which the number is greater than 2,500 millions per milliliter, wherein the viable bacteria comprise the streptomyces avermitilis, the paecilomyces lilacinus, the verticillium chlamydosporium and the Bacillus sphaericus. After the microbial preparation is prepared into soil, eggs of the root-knot nematode are killed by metabolites generated in the continuous growth and reproduction activity process of the viable bacteria, so that a granular structure of the soil is improved, the utilization rate of a fertilizer is improved and an effect of increasing both production and income is achieved.

Description

technical field [0001] The invention belongs to the field of biological control of crop pests, and relates to a microbial preparation for inhibiting root-knot nematodes, a preparation method and application thereof. Background technique [0002] Since root-knot nematode disease was first reported in southern my country in 1932, root-knot nematode disease has occurred in more than ten provinces and autonomous regions across the country. In recent years, vegetables in my country's protected areas have developed rapidly, and the same vegetable crops have been planted in greenhouses year after year, resulting in the increasing severity of root-knot nematode diseases in many old greenhouses, which has caused a substantial reduction in vegetable production and brought huge economic losses to vegetable farmers. Generally, 20%-25% of the economic loss is caused every year, and the serious loss is 50%-60%, or even production is lost. Therefore, the management of root-knot nematode d...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N63/04A01N63/02A01P5/00A01P21/00
Inventor 李峰
Owner 河北衡晓生物科技有限公司
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