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Stevia rebaudiana enzyme VI and method for converting rebaudioside-A into rebaudioside-D

A technology of stevia enzyme and stevia, applied in the direction of transferase, fermentation, etc.

Active Publication Date: 2013-04-10
SICHUAN INGIA BIOSYNTHETIC CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0012] So far, there is no report on the production of RD by enzymatic biotransformation, ultrafiltration and other technical methods using RA as raw material

Method used

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  • Stevia rebaudiana enzyme VI and method for converting rebaudioside-A into rebaudioside-D
  • Stevia rebaudiana enzyme VI and method for converting rebaudioside-A into rebaudioside-D
  • Stevia rebaudiana enzyme VI and method for converting rebaudioside-A into rebaudioside-D

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 4

[0054] Embodiment 4 investigates the impact of filtration, concentration method on solids RD content

[0055] After obtaining the reaction solution according to the conversion method of Example 1-18, if filter paper is used to filter and dry, solid A is obtained; if a 10kD ultrafiltration membrane is used for ultrafiltration, the ultrafiltration permeate is dried to obtain solid B; The ultrafiltrate was recrystallized and dried to obtain solid C. The HPLC detection method in the national standard GB8270-1999 was used to detect RD. The results are shown in Table 5.

[0056] table 5

[0057]

[0058] It can be seen from Table 5 that the rebaudioside D content in the solid A can reach at least 60%, and the further refined solid C red RD can reach 80%, or even more than 90%.

[0059] In summary, by applying the above enzymatic catalysis and separation methods, the conversion rate of rebaudioside D obtained from rebaudioside A is ≥ 70%, and the content of rebaudioside D in the ...

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Abstract

The invention belongs to the field of food chemistry, and particularly relates to a method for converting RA (rebaudioside-A) which is taken as a raw material into RD (rebaudioside-D), and the key is stevia rebaudiana enzyme VI and a preparation method thereof. The converting method comprises the following steps: A, the RA of which the purity is higher than 90% is dissolved with water according to the weight measurement ratio of 1:6-1:10m / v; B, UDP-Glu (Uridine Diphosphate Glucose) is added according to the weight ratio that RA:UDP-Glu=15:5-15:9; C, the stevia rebaudiana enzyme VI is added according to the weight ratio that RA: stevia rebaudiana enzyme VI=(800-1200):1 (preferably 1000:1), and then reaction is carried out for 3-7h at the temperature of 40-60 DEG C and the pH of 6-8; and D, a reaction solution is filtered and dried to obtain RD-containing solid matter. The conversion rate of converting RA into RD is larger than or equal to 70%, and the RD content in the solid matter is 60%-70%, can achieve 80% after further separation and purification, and even can be larger than 90%.

Description

technical field [0001] The invention belongs to the field of food chemical industry, and specifically relates to a method for converting rebaudioside A into rebaudioside D by using rebaudioside A as a raw material. The key lies in the catalyst steviolase VI and the preparation method thereof. Background technique [0002] Steviol glycoside is a general term for stevia extract, and its sweet components include stevioside (Stevioside), rebaudioside A, B, C, D, E (ebaudioside A / B / C / D / E), stevia Steviolbioside, Dulcoside A, etc. Stevia is native to South America and has been used for hundreds of years. The characteristics of high sweetness and low calorie of stevioside just meet the needs of modern people's life. The Ministry of Health of my country issued Wei Fang Zi No. 37 on June 5, 1985 (85), approving steviol glycosides as a new food additive. The World Health Organization's Joint Expert Committee on Food Additives (Joint FAO / WHO Expert Committee on Food AdditVIes, JECFA...

Claims

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Application Information

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IPC IPC(8): C12N9/10C12P19/56
Inventor 李沁桐华君欧阳劲秦岭
Owner SICHUAN INGIA BIOSYNTHETIC CO LTD
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