Method for increasing apomixis ratio of apple rootstocks and trimming degree of nursery stocks
An apomixis and uniformity technology, applied in botany equipment and methods, horticultural methods, horticulture, etc., can solve the problems of reduced proportion of apomixis seeds, great influence, unstable seedling uniformity and wide application
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Embodiment 1
[0012] In order to develop a technique to increase the rate of apomictic reproduction, this example first took the triploid Pingyi sweet tea as an example to study its apomictic characteristics, and the research comparison was the same triploid sexual reproduction cultivar Jona gold.
[0013] 1. 2N Embryo sac developmental retardation is a key developmental event in apomixis in Malus
[0014] Observation of developmental biology by laser confocal microscope found that compared with the same triploid sexual reproductive apple cultivar Jonagold, the development of the embryo sac of Pingyi Tiancha was seriously delayed. Sexual hybrids are formed after fertilization. Most of the embryo sacs of Pingyi sweet tea are far from mature at this time. After the fertilization process, the unfertilized 2N embryo sacs form asexual apomictic seeds through parthenogenesis.
[0015] Simultaneously, other apomictic Rosaceae plants such as Xiaojinhaitang and Shajinhaitang have also found simila...
Embodiment 2
[0028] Embodiment 2 The effect verification of the present invention
[0029] 1. The specific method steps of improving apple rootstock apomixis efficiency and seedling uniformity are as follows:
[0030] a. When the flowers of Pingyi Sweet Tea and Shajin Begonia have just entered the full flowering period (25% of the flowers are open), process them;
[0031] b. Spray the flowers in full flowering stage with 1.0 Baume degree of lime sulfur mixture, try to avoid spraying on the leaves;
[0032] c. Spraying method: Spray once every morning or evening (spray the flowers evenly until the petals are wet and the liquid does not drip), and spray for 3 consecutive days.
[0033] When lime sulfur mixture is sprayed on the stigmas of Pingyi Sweet Tea and Shajin Begonia flowers, the activity of the stigmas decreases and wilts quickly. At this time, the embryo sacs are immature and cannot complete the normal fertilization process. When mature, the stigma has wilted and lost the ability ...
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