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Method for increasing apomixis ratio of apple rootstocks and trimming degree of nursery stocks

An apomixis and uniformity technology, applied in botany equipment and methods, horticultural methods, horticulture, etc., can solve the problems of reduced proportion of apomixis seeds, great influence, unstable seedling uniformity and wide application

Inactive Publication Date: 2013-04-10
SHANDONG AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, these Malus plants all undergo facultative apomixis, and environmental factors such as temperature and light in the flowering period have a great influence on the apomixis rate. The variable flowering climate causes the apomixis rate to be unstable, resulting in The proportion of the seedlings is reduced, and the uniformity of the seedlings is greatly affected
Therefore, the instability of the uniformity of apomictic seedlings has become an obstacle limiting its wide application in production, and there is an urgent need to develop technologies to improve the rate of apomixis and the uniformity of seedlings

Method used

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  • Method for increasing apomixis ratio of apple rootstocks and trimming degree of nursery stocks
  • Method for increasing apomixis ratio of apple rootstocks and trimming degree of nursery stocks
  • Method for increasing apomixis ratio of apple rootstocks and trimming degree of nursery stocks

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Effect test

Embodiment 1

[0012] In order to develop a technique to increase the rate of apomictic reproduction, this example first took the triploid Pingyi sweet tea as an example to study its apomictic characteristics, and the research comparison was the same triploid sexual reproduction cultivar Jona gold.

[0013] 1. 2N Embryo sac developmental retardation is a key developmental event in apomixis in Malus

[0014] Observation of developmental biology by laser confocal microscope found that compared with the same triploid sexual reproductive apple cultivar Jonagold, the development of the embryo sac of Pingyi Tiancha was seriously delayed. Sexual hybrids are formed after fertilization. Most of the embryo sacs of Pingyi sweet tea are far from mature at this time. After the fertilization process, the unfertilized 2N embryo sacs form asexual apomictic seeds through parthenogenesis.

[0015] Simultaneously, other apomictic Rosaceae plants such as Xiaojinhaitang and Shajinhaitang have also found simila...

Embodiment 2

[0028] Embodiment 2 The effect verification of the present invention

[0029] 1. The specific method steps of improving apple rootstock apomixis efficiency and seedling uniformity are as follows:

[0030] a. When the flowers of Pingyi Sweet Tea and Shajin Begonia have just entered the full flowering period (25% of the flowers are open), process them;

[0031] b. Spray the flowers in full flowering stage with 1.0 Baume degree of lime sulfur mixture, try to avoid spraying on the leaves;

[0032] c. Spraying method: Spray once every morning or evening (spray the flowers evenly until the petals are wet and the liquid does not drip), and spray for 3 consecutive days.

[0033] When lime sulfur mixture is sprayed on the stigmas of Pingyi Sweet Tea and Shajin Begonia flowers, the activity of the stigmas decreases and wilts quickly. At this time, the embryo sacs are immature and cannot complete the normal fertilization process. When mature, the stigma has wilted and lost the ability ...

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Abstract

The invention provides a method for increasing the apomixis ratio of apple rootstocks and the trimming degree of nursery stocks. According to the method, before pollination and fertilization of apple flowers are finished, lime sulfur at the concentration of 1.0 baume degree is sprayed on the apple flowers at the full-blossom stage for one time in the morning or in the evening every day (the flowers are sprayed uniformly until the petals are wet and liquid does not drop off), continuously for three days. When the lime sulfur is sprayed on the apple flowers, the stigma activity is reduced, seriously, the stigma is wilted and the life of the stigma is shortened, so that the effective time of pollination and fertilization is shortened, the fertilization of the embryo sac is limited, the fertilization process of the 2N embryo sac of the apples with the apomictic feature is avoided, and the unfertilized 2N embryo sac forms clonal seeds through the apomixis way. By adoption of the lime sulfur, the life of the stigma is shortened and the pollination time is shortened, so that the apomixis ratio of the apple rootstock seeds with the apomictic feature and the trimming degree of the nursery stocks are increased.

Description

1. Technical field [0001] The invention relates to a method for improving the apomictic rate of apple stock and the uniformity of seedlings, belonging to the field of fruit tree seedling production. 2. Background technology [0002] Apple, one of the four major fruit trees in the world, is the main cultivated tree species in temperate regions; in fruit tree production, the tree body of cultivated fruit trees is a grafted complex of scion and rootstock, and rootstock is indispensable in the production of seedlings of fruit trees such as apples. At present, the commonly used rootstocks in fruit tree production mainly include seed rootstocks and clonal rootstocks. The seed stock generally comes from the hybrid seeds of wild apples (such as Octagonal Begonia and Huailai Begonia, etc.), and the genetic separation is serious, and the seedlings are not neat. Although the clonal rootstocks grow neatly, their siteability and resistance are poor. [0003] Apomixis of plants is a vari...

Claims

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Application Information

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IPC IPC(8): A01G7/06
Inventor 郝玉金由春香刘丹丹沙广利
Owner SHANDONG AGRICULTURAL UNIVERSITY
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