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Manufacturing method of ceramide and/or glucose ceramide production promoter

A technology of ceramide and manufacturing method, applied in the field of ceramide and/or glucosylceramide production accelerator, to achieve the effect of improving moisturizing ability

Inactive Publication Date: 2013-03-27
KAO CORP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] However, there is no report on the production of ceramide and / or glucosylceramide production promoters using microorganisms of the genus Hansenula (Hansenula)

Method used

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  • Manufacturing method of ceramide and/or glucose ceramide production promoter

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] (Manufacture of ceramide and / or glucosylceramide production promoter)

[0051] As a microorganism for producing ceramide and / or glucosylceramide production promoter from soybean dregs, Hansenula polymorpha (Hansenula polymorpha) (purchased from Jiangnan University and deposited in CICIM, China University Industrial Microbiology Resources and Information Center, deposit number CICIM Y0234).

[0052] Inoculate the above strain obtained by culturing in YPD (Yeast Extract Peptone Dextrose Medium) medium at 30°C into a Erlenmeyer flask (capacity 300ml) containing 100ml of ceramide / glucosylceramide production promoter production medium, and inoculate at 30°C cultured for 72 hours. The medium contained 10 w / v% okara and 1 w / v% glucose. Wherein, the preparation of soybean dregs is obtained by soaking soybeans in water for 12 hours to make soybeans fully absorb water, then grinding and crushing the soybeans that have absorbed water, filtering and removing liquid components, an...

Embodiment 2

[0056] As a microorganism for producing ceramide / glucosylceramide production accelerator from bean dregs, Hansenula polymorpha (purchased from Jiangnan University, and deposited in CICIM, China Universities Industrial Microbiology Resources and Information Center, preservation number CICIM Y0205 ). Using the same method as in Example 1 to obtain the fermented extract of soybean dregs, as the ceramide and / or glucosylceramide production accelerator of the present invention (product B-1 of the present invention), store it at 4°C and use it for subsequent neural Amide / glucosylceramide production promotion test.

Embodiment 3

[0058] As a microorganism for producing ceramide / glucosylceramide production accelerator from soybean dregs, Hansenula polymorpha (purchased from Jiangnan University and deposited in CICIM, China Universities Industrial Microbiology Resources and Information Center, accession number CICIMY0257) was used . Using the same method as in Example 1 to obtain the okara fermented extract, as the ceramide and / or glucosylceramide production accelerator of the present invention, further, dissolve the okara fermented extract with 50v / v% ethanol, and adjust its concentration to 0.1 After w / v% (invention product C-1), it was stored at 4° C. and used for the subsequent ceramide / glucosylceramide production promotion test.

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Abstract

The invention provides a manufacturing method of a ceramide and / or glucose ceramide production promoter, wherein the ceramide and / or glucose ceramide production promoter is obtained from supernatant fluid of a microorganism of Hansenula, which is cultured in a culture medium containing bean dregs and saccharides. According to the manufacturing method disclosed by the invention, the specific microorganism can be utilized, and a byproduct, namely the bean dregs in the food processing industry and the like can be used as a main raw material of the culture medium, so that the ceramide and / or glucose ceramide production promoter can be obtained with low cost and high efficiency.

Description

technical field [0001] The present invention relates to a method for producing a ceramide and / or glucosylceramide production promoter capable of promoting the production of ceramide and / or glucosylceramide in the skin by using specific microorganisms and using bean dregs, a by-product of the food processing industry, etc., as the main raw material of a culture medium . Background technique [0002] Ceramide, one of the sphingolipids, exists in the stratum corneum of the skin and accounts for about 50% of the intercellular lipids in the stratum corneum. Sphingolipid is a general term for complex lipids having a sphingosine skeleton. Glucosylceramide is a kind of sphingolipid formed by binding glucose to ceramide. The ceramide synthesized by epidermal cells is temporarily stored in the form of glucosylceramide or sphingomyelin; it is then excreted outside the cell, where glucocerebrosidase (Glucocerebrosidase) and sphingomyelinase (Sphingomyelinase) Under the action of cera...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P1/02A61K8/99A61Q19/00C12R1/78
Inventor 孔凡旗
Owner KAO CORP
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