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Formula of anabaena culture medium

A culture medium, technology of Anabaena, applied in the field of medium formula of Anabaena

Active Publication Date: 2014-06-11
LIYANG TECH DEV CENT
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are few reports about the culture method of Anabaena at present, and there are even fewer reports on the culture medium specially used for Anabaena

Method used

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  • Formula of anabaena culture medium

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0010] Prepare the culture medium with the following formula:

[0011] NH 4 NO 3 200mg / L, NaNO 3 500mg / L, K 2 SO 4 400mg / L, CaCl 2 .2H 2 O 150mg / L, MgSO 4 .7H 2 O 100mg / L, K 2 HPO 4 400mg / L, Na 2 S 2 o 3 .5H 2 0 15mg / L,H 3 BO 3 20mg / L, CuSO 4 .5H 2 O 2mg / L, Mo(NO 3 ) 3 .5H 2 O 1.5mg / L;

[0012] Ferric ammonium citrate 0.2mg / L, niacin 0.1mg / L, vitamin B 6 0.2mg / L, vitamin B 1 0.2mg / L, NNA0.05mg / L, IBA0.03mg / L.

[0013] Anabaena algae in the logarithmic growth phase were inoculated into the medium for cultivation, and the light was 10 hours a day, the temperature of the incubator was controlled at about 30 degrees Celsius, and the cell density of Anabaena in the medium was recorded every 2 days.

Embodiment 2

[0015] NH 4 NO 3 250mg / L, NaNO 3 550mg / L, K 2 SO 4 500mg / L, CaCl 2 .2H 2 O 200mg / L, MgSO 4 .7H 2 O 150mg / L, K 2 HPO 4 500mg / L, Na 2 S 2 o 3 .5H 2 0 20mg / L,H 3 BO 3 25mg / L, CuSO 4 .5H 2 O 4mg / L, Mo(NO 3 ) 3 .5H 2 O 2mg / L;

[0016] Ferric ammonium citrate 0.4mg / L, niacin 0.2mg / L, vitamin B 6 0.3mg / L, vitamin B 1 0.3mg / L, NNA 0.1mg / L, IBA 0.05mg / L.

[0017] Anabaena algae in the logarithmic growth phase were inoculated into the medium for cultivation, and the light was 10 hours a day, the temperature of the incubator was controlled at about 30 degrees Celsius, and the cell density of Anabaena in the medium was recorded every 2 days.

Embodiment 3

[0019] NH 4 N O 3 220mg / L, NaNO 3 520mg / L, K 2 SO 4 450mg / L, CaCl 2 .2H 2 O 170mg / L, MgSO 4 .7H 2 O 120mg / L, K 2 HPO 4 450mg / L, Na 2 S 2 o 3 .5H 2 0 17mg / L,H 3 BO 3 22mg / L, CuSO 4 .5H 2 O 3mg / L, Mo(NO 3 ) 3 .5H 2 O 1.7mg / L;

[0020] Ferric ammonium citrate 0.3mg / L, niacin 0.15mg / L, vitamin B 6 0.25mg / L, vitamin B 1 0.25mg / L, NNA0.07mg / L, IBA0.04mg / L.

[0021] Anabaena algae in the logarithmic growth phase were inoculated into the medium for cultivation, and the light was 10 hours a day, the temperature of the incubator was controlled at about 30 degrees Celsius, and the cell density of Anabaena in the medium was recorded every 2 days.

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Abstract

The invention provides a formula of an anabaena culture medium, comprising 200-250 mg / L NH4NO3, 500-550 mg / L NaNO3, 400-500 mg / L K2SO4, 150-200 mg / L of CaC12.2H2O, 100-150 mg / L MgSO4.7H2O, 400-500 mg / L K2HPO4, 15-20 mg / L Na2S2O3.5H2O, 20-25 mg / L H3BO3, 2-4 mg / L CuSO4.5H2O, 0.2-0.4 mg / L ammonium ferric citrate, 0.1-0.2 mg / L nicotinic acid, 0.2-0.3 mg / L vitamin B, 0.2-0.3 mg / L vitamin B1, 0.05-0.1 mg / L NNA and 0.03-0.05 mg / L IBA. The culture medium provided by the invention has the advantages of high survival rate and high growth rate while cultivating the anabaena, and are less prone to being polluted by other bacteria.

Description

technical field [0001] The invention relates to an algae culture medium, in particular to a culture medium formula of Anabaena. Background technique [0002] Anabaena blooms can fix free nitrogen in the atmosphere and are a type of nitrogen-fixing cyanobacteria. It grows and reproduces rapidly, and its nitrogen fixation capacity reaches 10-51kg / ha. After its algae sink into the soil, it can be absorbed by the roots of crops. A good organic fertilizer. The production and application of agricultural chemical fertilizers and pesticides will bring a large amount of greenhouse gas emissions. Therefore, the development of agricultural biological nitrogen fixation technology and the reduction of chemical fertilizer application are important ways to develop low-carbon agriculture. The uses of Anabaena blooms mainly include: extraction of natural pigments, phycobiliproteins and polysaccharides, research and utilization of algal toxins, biological nitrogen fixation and other aspects...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/12C12R1/89
Inventor 彭江晨芮旭婷
Owner LIYANG TECH DEV CENT
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