Preparation method of raspberry fermenting substance for cigarette
A technology of raspberry and fermented products, which is applied in the field of preparation of raspberry fermented products for tobacco, can solve the problems of changing the characteristic aroma of raspberry spices, unstable quality, precipitation, etc. gas effect
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Embodiment 1
[0023] 1) Culture medium preparation
[0024] a. Raw material pretreatment: raspberry and water are refluxed and extracted for 3 hours according to the weight ratio of 1:5, the extract is placed for 24 hours and then filtered, and the filtrate is collected to obtain the raspberry extract;
[0025] b. Adding glucose: adding 0.2% glucose by weight to the prepared raspberry extract to prepare the culture medium;
[0026] 2) Medium sterilization
[0027] Sterilize the medium at 115°C for 20 minutes;
[0028] 3) Strain preparation
[0029] Add the active dry acetic acid bacteria to 10 times the mass of normal saline, filter it with filter paper under sterile conditions, and take the filtrate as the bacteria.
[0030] 4) Vaccination
[0031] Under sterile conditions, the bacteria were inoculated into the culture medium with an inoculation amount of 0.05%;
[0032] 5) fermentation
[0033] The pH value is 6, the stirring speed is 50 rpm, anaerobic fermentation is carried out at...
Embodiment 2
[0038] 1) Culture medium preparation
[0039] a. Raw material pre-treatment: reflux extraction of raspberry and water according to the weight ratio of 1:10 for 3 hours, place the extract for 24 hours and then filter, collect the filtrate to obtain raspberry extract;
[0040] b. Adding glucose: adding 0.8% glucose by weight to the prepared raspberry extract to obtain a culture medium;
[0041] 2) Medium sterilization
[0042] Sterilize the medium at 115°C for 20 minutes;
[0043] 3) Strain preparation
[0044] Add the active dry acetic acid bacteria to 10 times the mass of normal saline, filter it with filter paper under sterile conditions, and take the filtrate as the bacteria.
[0045] 4) Vaccination
[0046] Under sterile conditions, the bacteria were inoculated into the culture medium with an inoculation amount of 0.1%;
[0047] 5) fermentation
[0048] The pH value was 7, the stirring speed was 100 rpm, anaerobic fermentation was carried out at 36° C. for 4 days, and...
Embodiment 3
[0053] 1) Culture medium preparation
[0054] a. Raw material pre-treatment: raspberry and water are refluxed and extracted for 3 hours according to the weight ratio of 1:15, the extract is placed for 36 hours and then filtered, and the filtrate is collected to obtain the raspberry extract;
[0055] b. Add glucose: add its weight 2% glucose to the prepared raspberry extract to prepare the culture medium;
[0056] 2) Medium sterilization
[0057] Sterilize the medium at 115°C for 20 minutes;
[0058] 3) Strain preparation
[0059] Add the active dry acetic acid bacteria to 10 times the mass of normal saline, filter it with filter paper under sterile conditions, and take the filtrate as the bacteria.
[0060] 4) Vaccination
[0061] Under sterile conditions, the strains were inoculated into the culture medium with an inoculation amount of 0.15%;
[0062] 5) fermentation
[0063] At a pH value of 7 and a stirring speed of 100 rpm, anaerobic fermentation was carried out at 3...
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