Coreopsis tinctoria active part and application thereof in preparation of whitening cosmetic
An active part, the technology of snow chrysanthemum, applied in the active part of snow chrysanthemum and its application in the preparation of whitening cosmetics, can solve the problems of literature and patent reports that have not found the whitening effect of snow chrysanthemum
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Embodiment 1
[0018] Snow chrysanthemum dry powder 20g, at room temperature (5 ~ 30 ℃) in 200ml of ethanol with a volume concentration of 30% cold soaking, ultrasonic extraction twice, each time 30min (500W), take the filtrate and combine, recover the solvent under reduced pressure until dry, take the extraction An appropriate amount of the substance was prepared into a 0.5mg / ml solution for later use, and its tyrosinase inhibitory effect was determined so as to compare with the activity of the active part of the snow chrysanthemum prepared later.
[0019] Result: IC of the tyrosinase inhibitory effect of the crude extract of Snow Chrysanthemum 50 1.85mg / ml.
Embodiment 2
[0021] Snow chrysanthemum dry powder 20g, at room temperature with 60% ethanol 200ml cold soaking, ultrasonic extraction 2 times, each time 30min (500W), take the filtrate and combine, recover the solvent under reduced pressure to dryness, take an appropriate amount of extract, and prepare 0.5 The mg / ml solution is ready for use, and its tyrosinase inhibitory effect is determined, so as to compare with the activity of the active part of the snow chrysanthemum prepared later.
[0022] Result: IC of the tyrosinase inhibitory effect of the crude extract of Snow Chrysanthemum 50 It was 1.07mg / ml.
Embodiment 3
[0024] Snow chrysanthemum dry powder 20g, at room temperature with 70% ethanol 200ml cold soaking, ultrasonic extraction 2 times, each time 30min (500W), take the filtrate and combine, recover the solvent under reduced pressure to dryness, take an appropriate amount of extract, and prepare 0.5 The mg / ml solution is ready for use, and its tyrosinase inhibitory effect is determined, so as to compare with the activity of the active part of the snow chrysanthemum prepared later.
[0025] Result: IC of the tyrosinase inhibitory effect of the crude extract of Snow Chrysanthemum 50 It is 0.92mg / ml.
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