Data analysis method for electrical impedance type quick bacteria detection sensor

A detection sensor and data analysis technology, applied in the direction of material impedance, can solve complicated, time-consuming and labor-intensive problems, and achieve the effect of reducing interference

Inactive Publication Date: 2013-03-06
SHANGHAI INST OF MICROSYSTEM & INFORMATION TECH CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

This traditional method of culture, isolation and biochemical identification relying on culture medium is time-consuming, laborious and complicated.

Method used

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  • Data analysis method for electrical impedance type quick bacteria detection sensor
  • Data analysis method for electrical impedance type quick bacteria detection sensor
  • Data analysis method for electrical impedance type quick bacteria detection sensor

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] The making of interdigitated electrode in step 1 of embodiment 1

[0021] An interdigitated array (IDA) is a pair of electrode arrays interdigitated with each other. The distance between the finger-shaped sub-electrodes is in the order of microns or nanometers, and a small amount of liquid to be tested can be used to obtain desired results. As the size of the electrode decreases, the frequency corresponding to different impedance regions decreases accordingly, and the change of impedance before and after bacterial growth can be measured at a lower frequency. Compared with a simple counter electrode, the IDA electrode has a smaller size and a larger surface area, so that bacteria have a higher chance of adhering to the electrode surface, and the measurement task can be completed in a shorter time with less liquid.

[0022] A patterned Au metal thin layer is formed on the glass sheet using the lift-off process. The schematic diagram of the structure is shown in figure 1...

Embodiment 2

[0023] Influencing factors of embodiment 2 electrode parameters

[0024] The parameters of the electrode are critical to testing the sensitivity of the electrode system. The electrode material, thickness, interdigital logarithm, and the ratio of interdigital spacing and width of the electrode will all affect the sensitivity of the test system. Experiments have found that the strength of the signal is related to the surface area of ​​the entire electrode array, and the reduction of the electrode width will increase the signal-to-noise ratio.

[0025] The thickness of the sputtered Au layer is 0.1um, the relationship between the finger electrode width and the sensitivity is tested under the conditions that the ratio of inter-finger spacing to width is 1:1, the number of interdigital pairs is 20, and the length of interdigital electrodes is 2mm. Set the width of the finger electrode to 20um, 25um, 30um, 50um, 100um, 200um. The experimental results preliminarily prove that under...

Embodiment 3

[0030] When the measuring electrode is added to the sample, the electric double layer at the interface between the electrode and the solution is generally equivalent to a capacitor, called electric double layer capacitance, so the frequency response characteristics of the double layer capacitance described above are not consistent with "pure capacitance" , there is a "diffusion effect". The equivalent element is the constant phase angle element (CPE) Q. The equivalent model of this electrode testing system is the series connection of the equivalent constant phase angle element Q and the equivalent resistance R. Its impedance is:

[0031]

[0032] Y is the parameter of the equivalent element Q, its dimension is Ω -1 cm -2 ·s -n ; n is a dimensionless index, and 0 And the line that intersects the real axis at R (such as image 3 shown).

[0033] Although the frequency response characteristics of electric double-layer capacitance and "pure capacitance" have greater or le...

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Abstract

The invention relates to a data analysis method for an electrical impedance type quick bacteria detection sensor. According to the data analysis method, based on the electrical impedance principle, the sensor designs and manufactures an interdigital electrode for quickly determining bacteria by using the MEMS (Micro-Electromechanical System) technology; impedance and phase signals collected by the interdigital electrode are processed to obtain a Nyquist curve; the number of the bacteria is well represented by the slope of the Nyquist curve; and the slope change of the Nyquist curve in a bacterial growth process is approximately in a linear relation, thereby establishing a relation between the parameter value and the original bacterium quantity in a sample so as to determine the types and quantity of the bacteria. According to the data analysis method, the requirements on a testing electrode and a measurement environment are reduced, the time for determining the bacteria is shortened, and the data analysis method can be used for quickly detecting microbes.

Description

technical field [0001] The invention relates to a data analysis method of an electrical impedance type bacteria rapid detection sensor. The method can be used in rapid detection of microorganisms. Background technique [0002] Rapid detection of viable bacteria is a challenge. The commonly used method is done in petri dishes filled with agar. The number of bacteria generally doubles every 20-40 minutes, so it takes a long time to reach the number of bacteria in the colony required for detection. This traditional method of culture, isolation and biochemical identification relying on culture medium is time-consuming, laborious and complicated. In order to quickly, conveniently and correctly test food microorganisms, in recent years, many countries have conducted research on this and made progress. [0003] The microbial impedance detection method is a rapid detection method for qualitative and quantitative analysis of microorganisms by measuring the changes in electrical i...

Claims

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Application Information

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IPC IPC(8): G01N27/02
Inventor 金庆辉张凤金妍葛玉卿赵建龙
Owner SHANGHAI INST OF MICROSYSTEM & INFORMATION TECH CHINESE ACAD OF SCI
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