Method for preparing chlorella by high cell density fermentation

Abstract

The invention discloses a method for preparing chlorella by high cell density fermentation. The method comprises the following steps: firstly mounting a waterproof and explosion-proof illuminating lamp in a fermentation tank; performing activation and culture on an L tube strain containing 5ml of solid culture medium, then illuminating liquid in a conical flask and shaking the flask for amplification culture; further inoculating the strain after amplification culture into a fermentation culture medium with illumination in a full-automatic liquid fermentation tank for ventilation, fermentation and culture, feeding complementary carbon and feeding complementary nitrogen during the period, always keeping the concentration of glucose in fermentation liquid at 20% and keeping the concentration of NH4NO3 at 15%, fermenting till 60 hours, and stopping material supplementation; enabling the total fermentation time to be 72 hours; and centrifugating a culture solution after the end of fermentation, and abandoning supernatant fluid to get the chlorella. The chlorella is prepared by utilizing an autotrophy and heterotrophy parallel fermentation method disclosed by the invention in a high cell density manner, the process is simple, the fermentation period is short, the chlorella can keep integral nutritional ingredients, and the scale production of the chlorella in the fermentation tank by a heterotrophy method further becomes possible.

Description

technical field [0001] The invention relates to a method for preparing chlorella by high cell density fermentation, which belongs to the field of biological technology. technical background [0002] As an important microalgae resource, Chlorella is extremely rich in nutrients and has excellent health care effects. It is rich in protein, amino acid, pigment, polyunsaturated fatty acid, etc., and has comprehensive nutrition. It is an ideal feed material for aquatic products and animal husbandry. Its protein content is 50% to 67%, which contains 20 kinds of amino acids necessary for the human body, various vitamins and trace elements, as well as linolenic acid, linoleic acid, carotenoids and other ingredients. Chlorella is rich in Chlorella Growth Factor (CGF), which can quickly restore the damage caused by the organism of farmed animals. The biologically active substances glycoproteins, polysaccharides and up to 13% of nucleic acids contained in Chlorella have significant ac...

AI Technical Summary

Problems solved by technology

At present, the reasons for restricting the application of chlorella in marine aquaculture include: low nutrient content of algae species, high output cost, technical bottlenecks such as control of bacterial pollution under large-scale cultivation have yet to be broken through

Most of the production methods currently used are track-type culture ponds or bag-type photosynthetic culture. This culture method is greatly affected by climatic conditions (temperature, light, etc.), and is easily attacked by predators (rotifers, ciliates, etc.). Farming yield is low and difficult to stabilize