Method for extracting total magnolol according to alcoholic-alkaline method

Abstract

The invention provides a method for extracting total magnolol according to an alcoholic-alkaline method. The method comprises the following steps: firstly drying magnolia bark, smashing the magnolia bark and screening the magnolia bark through a 100 mesh screen, then preparing alcoholic-alkaline solution with 0.01 to 0.025N alkalinity through 50 to 70 percent alcoholic solution, mixing the magnolia bark powder and the alcoholic-alkaline solution according to the ratio of sample to solution (g / ml) being 1:10 to 1:20, performing ultrasonic extraction for 20 to 40 min, performing centrifugal separation, obtaining magnolia bark residue and alcoholic-alkaline solution I containing total magnolol, performing ultrasonic extraction again, concentrating the alcoholic-alkaline solution, adjusting the pH value of the concentrated solution to 2 to 3 through hydrochloric acid, refrigerating the concentrated solution for 6 to 8h at 2 to 6 DEG C, obtaining sediment, washing the sediment through water to be neutral, and the like. The method extracts the total magnolol according to the alcoholic-alkaline method by combining magnolol and physical and chemical properties of the magnolol, and has the advantages that the extraction efficiency is high, the energy consumption is low, the production is reduced, and the method is simple and efficient.

Description

technical field [0001] The invention belongs to the field of extracting plant active ingredients, and combines the physical and chemical properties of magnolol and honokiol to extract total magnolol, specifically a method for extracting total magnolol by an alcohol-alkali method. [0002] Background technique [0003] The traditional Chinese medicine Magnolia officinalis is a plant of the genus Magnolia in the family Magnoliaceae ( Magnolia officinalis Rehd. et Wils .) and its variant Magnolia officinalis ( Magnolia officinalis Rehd. et Wils. var. biloba Rehd. et Wils .) dry bark, branch bark and root bark, its active ingredients mainly include magnolol, magnolol, honokiol, alkaloids and volatile oil, etc., and also rich in amino acids, vitamins and other nutrients. Modern pharmacological studies have shown that magnolol and honokiol have analgesic, anti-inflammatory, antibacterial, anti-tumor, anti-ulcer, nervous system protection, and anti-oxidation effects. [0004]...

AI Technical Summary

Problems solved by technology

Alkaline extraction and acid precipitation method extract Magnolia officinalis mainly adopts dipping method, dipping method, and its extraction time is long, extraction efficiency is low, such as the application number is 200710035596.9, the name is " the efficient extraction method of magnolol and honokiol crude extract "; adopt ethanol reflux extraction method, although its extraction efficiency is high, will consume a large amount of energy, increased production cost, as application number is 200910069565.4, title is called " a kind of method of changing the viscosity of magnolia bark extract "; supercritical CO 2Extraction method has high extraction efficiency and good extraction purity of Magnolia officinalis, but the equipment is expensive and the extraction amount is small, so it is rarely used in actual production