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Human hard tissue repair material and preparation method thereof

A technology for repairing materials and hard tissue, applied in prosthesis, medical science, etc., can solve the problems of high cost, complex process, difficult to mass production, etc.

Inactive Publication Date: 2015-01-14
CENT SOUTH UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, recombinant human bone morphogenetic protein-2 (rhBMP-2) prepared by genetic engineering technology has been approved by the U.S. FDA and has begun to be used clinically. At the same time, there are potential safety issues of genetic engineering products [2]

Method used

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  • Human hard tissue repair material and preparation method thereof
  • Human hard tissue repair material and preparation method thereof

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Experimental program
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Effect test

Embodiment 1

[0038] Embodiment 1 The preparation method of bone repair material according to the present invention 1

[0039] The peptide dry powder shown in SEQ ID NO: 1-10 is dissolved in deionized water, and the concentration is 20 mg / mL. Take hydroxyapatite particles and polypeptide solution, the mass ratio of polypeptide to hydroxyapatite is 10:1, mix evenly at 25 degrees Celsius for 60 minutes, and then obtain the bone repair material of the present invention.

Embodiment 2

[0040] Embodiment 2 The preparation method of bone repair material according to the present invention 2

[0041] Peptide dry powder shown in SEQ ID NO: 1-10 was dissolved in deionized water at a concentration of 1 mg / mL. Take the peptide liquid drop on the hydroxyapatite sheet, let it stand at 25 degrees Celsius for 60 minutes, and then wash off the unresolved residue with PBS solution. The adsorbed polypeptide can obtain the bone repair material of the present invention. The mass ratio of polypeptide to hydroxyapatite is 1:1000.

Embodiment 3

[0042] Embodiment 3 The preparation method of bone repair material according to the present invention 3

[0043] Peptide dry powder shown in SEQ ID NO: 1-10 was dissolved in deionized water at a concentration of 0.05 mg / mL. The hydroxyapatite block was placed in the polypeptide liquid, and subjected to negative pressure adsorption at 37 degrees Celsius for 60 minutes to obtain the present The bone repair material described in the invention. The mass ratio of polypeptide to hydroxyapatite is 1:10.

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Abstract

The invention belongs to the field of a biomedical material, which is mainly applied to the preparation of a compound dosage form both of bone morphogenetic protein 2 active peptide and hydroxyapatite, wherein a sequence of the bone morphogenetic protein 2 active peptide is represented by SEQ ID NO: 1-10. A preparation method provided by the invention comprises the following steps: dissolving the bone morphogenetic protein 2 active peptide into normal saline or 5% of glucose solution, and then adding a hydroxyapatite support, combining the bone morphogenetic protein 2 active peptide on the surfaces of the hydroxyapatite particles to obtain the necessary compound dosage form both of the bone morphogenetic protein and hydroxyapatite after centrifugal separation, washing and drying, so as to obtain a human hard tissue repair material provided by the invention.

Description

technical field [0001] The invention relates to a human body hard tissue repair material for stimulating bone growth, and belongs to the field of medical and biological inventions. Background technique [0002] The clinical incidence of bone defect is high, and its treatment still lacks satisfactory bone repair materials. An ideal bone repair material should have the characteristics of biocompatibility, osteoconductivity, osteoinductivity, and osteogenesis. Due to the above-mentioned characteristics of autologous bone, it is still common to treat bone defects with autologous bone transplantation in clinical practice. Bone transplantation is the "gold standard" for treating bone defects, but autologous bone grafts have a limited supply of bone, and the operation time is long, causing damage to the donor site tissue, and complications such as donor site injury and pain are as high as 25% to 30%. Although allogeneic bone transplantation avoids the damage caused by autologous b...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L27/46A61L27/56A61L27/22
Inventor 黄智于博周科朝张斗李志友刘正春
Owner CENT SOUTH UNIV
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