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Human hard tissue repair material and preparation method thereof

A technology for repairing materials and hard tissues, applied in prosthesis, medical science, etc., can solve problems such as difficult large-scale production, high cost, and complicated process

Inactive Publication Date: 2013-01-23
CENT SOUTH UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, recombinant human bone morphogenetic protein-2 (rhBMP-2) prepared by genetic engineering technology has been approved by the U.S. FDA and has begun to be used clinically. At the same time, there are potential safety issues of genetic engineering products [2]

Method used

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  • Human hard tissue repair material and preparation method thereof
  • Human hard tissue repair material and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Example 1 Preparation method 1 of the bone repair material of the present invention

[0039] The polypeptide dry powder shown in SEQ ID NO: 1-10 is dissolved in deionized water, and the concentration is 20 mg / mL. Take the hydroxyapatite particles and the polypeptide liquid, the mass ratio of the polypeptide to the hydroxyapatite is 10:1, and the mixture is mixed at 25 degrees Celsius for 60 minutes to obtain the bone repair material of the present invention.

Embodiment 2

[0040] Example 2 Preparation method 2 of the bone repair material of the present invention

[0041] The polypeptide dry powder shown in SEQ ID NO: 1~10 was dissolved in deionized water with a concentration of 1 mg / mL. Take the polypeptide drop on the hydroxyapatite tablet, let it stand for 60 minutes at 25 degrees Celsius, and then wash it with PBS solution. The adsorbed polypeptide obtains the bone repair material of the present invention. The mass ratio of polypeptide to hydroxyapatite is 1:1000.

Embodiment 3

[0042] Example 3 Preparation method 3 of the bone repair material of the present invention

[0043] The polypeptide dry powder shown in SEQ ID NO: 1~10 is dissolved in deionized water at a concentration of 0.05 mg / mL. Place the hydroxyapatite block in the polypeptide solution and absorb under negative pressure at 37 degrees Celsius for 60 minutes to obtain the The bone repair material of the invention. The mass ratio of polypeptide to hydroxyapatite is 1:10.

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Abstract

The invention belongs to the field of a biomedical material, which is mainly applied to the preparation of a compound dosage form both of bone morphogenetic protein 2 active peptide and hydroxyapatite, wherein a sequence of the bone morphogenetic protein 2 active peptide is represented by SEQ ID NO: 1-10. A preparation method provided by the invention comprises the following steps: dissolving the bone morphogenetic protein 2 active peptide into normal saline or 5% of glucose solution, and then adding a hydroxyapatite support, combining the bone morphogenetic protein 2 active peptide on the surfaces of the hydroxyapatite particles to obtain the necessary compound dosage form both of the bone morphogenetic protein and hydroxyapatite after centrifugal separation, washing and drying, so as to obtain a human hard tissue repair material provided by the invention.

Description

Technical field [0001] The invention relates to a human hard tissue repair material used to stimulate bone growth, and belongs to the field of medical biology inventions. Background technique [0002] Bone defect has a high clinical incidence, and its treatment still lacks satisfactory bone repair materials. The ideal bone repair material should have the characteristics of biocompatibility, osteoconductivity, osteoinductivity, and osteogenic properties. Because autologous bone has all the above characteristics, it is still common in clinical practice to treat bone defects with autologous bone transplantation. Bone transplantation is the "gold standard" for the treatment of bone defects, but autologous bone transplantation has limited bone supply and long operation time, causing damage to the donor site tissues, and complications such as donor site injury and pain as high as 25% to 30%. Although allogeneic bone transplantation avoids the damage caused by autologous bone transplan...

Claims

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Application Information

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IPC IPC(8): A61L27/46A61L27/56A61L27/22
Inventor 黄智于博周科朝张斗李志友刘正春
Owner CENT SOUTH UNIV
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