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Pichia anomala

An abnormal Pichia pastoris, application technology, applied in the field of microorganisms, can solve the problem that the amount of ester production cannot meet the production requirements, and achieve the effect of strong ester production ability and good passability

Active Publication Date: 2014-01-22
SICHUAN JIANNANCHUN GRP CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although some enterprises and research institutions have conducted research on the production of EA flavor substances, and obtained some special functional strains with high ethyl acetate production, compared with traditional Daqu, although their ester production ability has been improved, but overall The amount of ester produced still cannot meet the production requirements

Method used

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Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0023] The saccharification and fermentation equipment (saccharification tank, jet liquefier, vacuum flash cooling system, etc.) used in the preparation of saccharification liquid in the following examples were all designed and manufactured by Yibin Minjiang Group Minjiang Machinery Factory.

[0024] The gas chromatograph in the following examples is Agilent 6890GC, the chromatographic column is HP-INNOWAX, the detector temperature is 280°C, the injection port temperature is 260°C, the split ratio is 20:1, and the temperature program is maintained at 40°C 8min, then raised to 100°C at a rate of 5°C / min, then raised to 220°C at a rate of 15°C / min, and kept for 8 minutes.

[0025] The culture media used in the following examples belong to the purchased synthetic culture media. Both the wort culture medium and the potato culture medium are products of Beijing Aoboxing Biological Co., Ltd., the product number of the wort culture medium is 02-183, and the potato culture medium The ...

Embodiment 1

[0026] Embodiment 1 natural selection

[0027] The following strains are all functional strains with high ethyl acetate production isolated and purified from koji and fermented grains of Jiannanchun Group Company. The specific process is as follows:

[0028] ① Collection of samples:

[0029] Koji medicine samples were collected from Luzhou-flavored Daqu with a storage period of 2 to 3 months. More than 10 half pieces of koji bricks (different storage rooms) were selected and crushed and mixed to obtain a comprehensive sample of koji medicine. The crushing fineness is able to pass through a 20-mesh sieve (that is, the sieve with 20 holes / cm 3 ) fine powder content accounts for 80% of the total amount of Quyao.

[0030] The fermented grains are selected from the middle and upper layers of high-quality female grains (exit pit grains). Take five points, that is, the four corners and the central part of the fermented grains. Take 200g from each point and mix them as a sub-sample...

Embodiment 2

[0033] Embodiment 2 production performance test

[0034] Preparation of bacterial suspension: Dip a little distilled water with a sterile cotton swab and let the cotton swab soak. Gently swirl the swab over the purified colony so that the cotton tip is covered with bacteria. Quickly insert the cotton swab covered with bacteria into the turbidimetric tube filled with 20mL sterile water. Rotate the cotton swab to disperse the bacteria in the sterile water, from the inside to the outside, from slow to fast, rotate and draw out the cotton swab. Shake the bacterial suspension on a vortex shaker and insert it into the cell concentration meter. Continue to use this method or add sterile water to adjust the bacterial concentration of various bacterial suspensions to 10 6 individual / mL.

[0035] Fermentation: Take 2mL of bacterial suspension and 3mL of absolute ethanol, add them to the prepared 100mL wort medium (115°C, sterilize for 20min), keep the temperature at 30°C, aerobic, a...

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Abstract

The invention relates to pichia anomala, and belongs to the field of microbes. The invention provides a pichia anomala strain for producing ethyl acetate in high yield. According to the pichia anomala JNC-EA002, the conservation number is CGMCC No. 5955. The invention also provides application of the strain to the production of seasoning spirits. The pichia anomala strain is high in ester producing capacity and is suitable for the large-scale production of the seasoning spirits.

Description

technical field [0001] The invention relates to Pichia anomaly and belongs to the field of microorganisms. Background technique [0002] High-grade liquor is very popular among consumers, but its price is increasing year by year because of its high quality and low quantity. A large number of liquors below high grade need to be blended with seasoning wines with high content of aroma and taste substances, so as to reach a higher quality level and meet the corresponding quality standards. [0003] The traditional seasoning wine production is carried out in the process mode of solid-state and semi-solid-state fermentation of pure grains. Generally, Daqu is used as a saccharification starter, and various bacteria, yeasts, molds and various enzymes (amylase, fruit, etc.) in Daqu are used. Glue enzyme, cellulase, acid protease and other enzymes) work together to obtain the target product. However, the production of modern flavored wine tends to be fermented by pure strains gradua...

Claims

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Application Information

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IPC IPC(8): C12N1/16C12P7/62C12G3/02C12R1/84
Inventor 谢义贵唐胜春石永凌
Owner SICHUAN JIANNANCHUN GRP CO LTD
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