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Antifreeze protein

A protein and substance technology, applied in the field of antifreeze protein

Inactive Publication Date: 2013-01-09
KANEKA CORP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the antifreeze protein obtained by the method disclosed in Patent Document 3 does not necessarily have sufficient antifreeze activity

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0097] Embodiments of the present invention will be described in more detail below by showing examples. Of course, the present invention is not limited to the following examples, and the details may have various forms. Further, the present invention is not limited to the above-mentioned embodiments, and various changes can be made within the scope shown in the claims, and embodiments obtained by appropriately combining various disclosed technical means are also included in the embodiments of the present invention. within range. In addition, all patent documents and non-patent documents described in this specification can be cited in this specification as a reference.

[0098] Manufacturing Example 1-1 Extraction

[0099] 300 mg of dried radish seedling extract powder was dissolved in 60 ml of Tris-HCl (5 mM, pH 8.0) to obtain a crude extract.

manufacture example 1-2

[0100] Manufacturing Example 1-2 Centrifugal Separation

[0101] 60 ml of the crude extract obtained in Production Example 1-1 was placed in a 300 ml beaker, heat-treated in a water bath (manufactured by EYELA) at 50° C. for 30 minutes, and then centrifuged for 15 minutes (10,000×g). The supernatant after centrifugation was recovered.

manufacture example 1-3

[0102] Production Example 1-3 Grading of Acetone

[0103] To 56 ml of the centrifuged supernatant obtained in Production Example 1-2, acetone cooled to -30° C. was added dropwise at a time until the final concentration was 30% by volume. Centrifugation was performed for 15 minutes (10,000×g), and the precipitate was recovered. The resulting precipitate was lyophilized and dissolved in 6 ml of Tris-HCl (5 mM, pH 8.0).

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Abstract

Provided is an antifreeze protein which is capable of being efficiently and inexpensively produced on an industrial level, is safe, and has excellent antifreeze activity for use on a practical level. Also provided are: a polypeptide that is the active segment of the antifreeze protein; a composition, food product, biological sample preservative and cosmetic comprising the antifreeze protein or polypeptide; and an antibody that specifically reacts with the antifreeze protein or polypeptide. The antifreeze protein is plant-derived and has a predetermined amino acid sequence, or is a plant seed protein.

Description

technical field [0001] The present invention relates to antifreeze protein, polypeptide as its active part, composition comprising said antifreeze protein or polypeptide, food, biological sample protection agent and cosmetics, and antibody specifically reacting with said antifreeze protein or polypeptide . Background technique [0002] Antifreeze proteins are substances that are also called antifreeze proteins or AFPs (antifreeze proteins). Conventional antifreeze proteins exhibit effects of inhibiting ice crystallization and controlling ice crystal shape, etc., accompanied by thermal hysteresis activity. Here, thermal hysteresis refers to the temperature range in which ice cannot grow even at a temperature below the equilibrium melting point in an aqueous protein solution. If the freezing point is defined as the temperature at which ice begins to grow in an aqueous solution, thermal hysteresis is defined as the equilibrium melting point and The difference between freezing...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K4/10A61K8/64C07K16/16C09K3/00A23L1/30
CPCC09K3/185A23V2002/00A61Q19/00A61K8/645A61K2800/52A23J3/14A23L1/3055A23L3/36A23V2200/206A23V2250/548
Inventor 福冈让一河原秀久毛笠秀昭荒井直树友野润横田真一
Owner KANEKA CORP
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