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Molecular marker for detecting susceptibility of ovarian cancer

A technology for ovarian cancer and susceptibility, applied in the field of de polymor, can solve the problems of no GADD45A gene, no GADD45A gene correlation, etc., and achieve the effect of strong specificity and simple detection

Active Publication Date: 2014-04-16
SHANDONG UNIV QILU HOSPITAL
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] In the prior art, although more than 200 candidate genes and 1100 genetic variations in 20 genetic regions have been associated with ovarian cancer susceptibility, there are very few genetic variations that are strongly associated with ovarian cancer
And there is no report on the correlation between GADD45A gene and ovarian cancer, and there is no report on the correlation between GADD45A gene SNP and ovarian cancer

Method used

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  • Molecular marker for detecting susceptibility of ovarian cancer
  • Molecular marker for detecting susceptibility of ovarian cancer
  • Molecular marker for detecting susceptibility of ovarian cancer

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Example 1 Detection of GADD45A gene mutation and association analysis with ovarian cancer

[0020] 1.1 Research object

[0021] A total of 139 patients with ovarian cancer were selected and diagnosed and treated in Qilu Hospital of Shandong University from September 2008 to April 2011. The clinical data were obtained from medical records. 189 age-matched control patients were healthy women who underwent physical examination in the Health Examination Center of Qilu Hospital. Most of the subjects were Han residents in Shandong Province. Take 1.5ml of peripheral blood from the test personnel and store it in a freezer at -80°C. All subjects obtained consent in accordance with the requirements of the Ethics Committee of Qilu Hospital of Shandong University.

[0022] 1.2 DNA extraction

[0023] The peripheral blood DNA of the subjects was extracted by the conventional phenol-chloroform method, as follows:

[0024] (1) Take 400ul of blood and add it to a 1.5ml centrifuge ...

Embodiment 2

[0047] Example 2 Ovarian Cancer Susceptibility Detection Kit

[0048] Since the 1506T>C mutation in SEQ ID NO: 1 is highly related to the publication of ovarian cancer, GADD45A gene-specific primers can be designed based on this mutation and then expanded detection using the patient's DNA as a template.

[0049] Prepare a kit (100 person-times), which contains the substances shown in Table 3:

[0050] table 3

[0051]

[0052] 2ml of peripheral blood was drawn from the subjects, and DNA was extracted by conventional methods. Ovarian cancer susceptibility detection kit was used to carry out PCR reaction, the reaction products were sequenced, and the sequencing results were checked and analyzed using Meglign7.0 and Chromas2.33 software. The susceptibility of subjects with 1506T>C in the test results to ovarian cancer is higher than that of the normal population.

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Abstract

The invention discloses a molecular marker for detecting susceptibility of ovarian cancer. Nucleotide sequences of the molecular marker are shown in SEQ ID NO.1, and mononucleotide polymorphism, namely T>C is present at 1506th site. A kit for detecting susceptibility of ovarian cancer comprises a pair of specific primers having sequences in SEQ ID NO.2 and SEQ ID NO.3. A method for detecting presence of mononucleotide polymorphism in GADD45A (growth arrest and DNA-damage-inducible protein 45 alpha) gene in a sample in vitro includes: firstly, amplifying the GADD45A gene in the sample by the specific primers to obtain an amplification product; secondly, subjecting the amplification product to sequence determination, and detecting presence of mononucleotide polymorphism, namely 1506T>C, in the amplification product. The method is simple, accurate and fast in detection and high in specificity, and is important to effectively implementing early diagnosis, early treatment and individual prevention and treatment to high risk groups or susceptible individuals and patients with ovarian cancer.

Description

technical field [0001] The present invention relates to a molecular marker, a kit and a detection method for detecting the susceptibility of ovarian cancer, in particular to the single nucleotide polymorphism (single nucleotide polymorphism, SNP) of GADD45A gene and its correlation with ovarian cancer The properties, and methods and kits for detecting these SNPs belong to the fields of molecular biology and medicine. Background technique [0002] Ovarian cancer is the most lethal tumor of the female reproductive system. In 2008, there were 225,500 new cases and 140,200 deaths worldwide. Due to insidious onset and inconspicuous symptoms, most patients are diagnosed at an advanced stage. The 5-year survival rate of patients with advanced ovarian cancer is only about 30-45%, and the prognosis is poor. However, the 5-year survival rate of patients with early ovarian cancer is as high as 90%. It reminds us that the key to improving the prognosis and survival of ovarian cancer pa...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/68C12N15/11
Inventor 孔北华苑存忠杨其峰闫实刘晓燕杨宁
Owner SHANDONG UNIV QILU HOSPITAL
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