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Livestock and poultry excrement microbial deodorizing agent, and preparation method and application thereof

A deodorant bacterium agent and microbial technology, applied in the field of microbial deodorizer bacterium agent and its preparation, can solve problems such as small application range, secondary pollution, and deodorization efficiency failure, so as to improve immunity, prolong service life, and ensure The effect of food safety

Active Publication Date: 2013-01-02
CHENGDU INST OF BIOLOGY CHINESE ACAD OF S
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, the research on the deodorization of livestock and poultry manure can be divided into two aspects: chemical deodorization and biological deodorization. Strong, quick effect, but its application range is small, when encountering complex odorous gas components, the deodorizing effect will be invalid, and the residual chemical substances after using chemical deodorants often cause secondary pollution

Method used

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  • Livestock and poultry excrement microbial deodorizing agent, and preparation method and application thereof
  • Livestock and poultry excrement microbial deodorizing agent, and preparation method and application thereof
  • Livestock and poultry excrement microbial deodorizing agent, and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] (1) Quantitatively prepare the high-temperature sterilized (121°C, 30 minutes) YPD solid medium for use. The formula of the solid YPD medium is 20g of peptone, 10g of beef extract, 20g of glucose, 1000mL of water, 15-20g of agar, pH 6.5-7.0.

[0047] (2) Introduce strains CR and NR into sterilized (121°C, 30 minutes) YPD solid slant medium for activation respectively.

[0048] (3) Quantify the liquid medium that has been sterilized at high temperature (121°C, 30 minutes) for later use. The formula of the liquid medium is 5g of peptone, 10g of malt extract, 20g of glucose, 1000mL of water, and pH 6.5-7.0.

[0049] (4) Put the activated strains into the liquid medium and shake (30°C, 160 rpm) for 24 hours.

[0050] (5) Count the cultured bacterial solution, and adjust the bacterial content of the second strain to 10 8 CFU / mL, according to the volume ratio of CR: NR = 2: 1, it is combined into a mixed bacterial liquid, which is a liquid microbial deodorant bacterial agen...

Embodiment 2

[0054] Add 10ml of liquid microbial deodorizing agent to a beaker (1L) containing 200g of fresh pig manure, and seal the beaker with plastic wrap; at the same time, do a control without adding agent, and set three repetitions for each group of experiments. After 15 days, measure the odor intensity, Ammonia concentration, hydrogen sulfide concentration, measurement results are as follows in Table 1:

[0055] Table 1: Deodorizing effect of bacteria agent

[0056]

[0057] It can be seen from the above table that after adding bacteria agents to pig manure, the odor intensity, ammonia concentration and hydrogen sulfide concentration were significantly lower than those of the control, among which the odor intensity decreased by 38.8%, the ammonia concentration decreased by 44.3%, and the hydrogen sulfide concentration decreased by 33.8%. %.

Embodiment 3

[0059] Add 100g of solid microbial deodorizing agent (after activation) to a beaker (2L) filled with 1000g of fresh pig manure, mix well with the pig manure, and seal the beaker with plastic wrap; at the same time, make a control without adding the agent, each group of experiments Three repetitions were set, and the stench intensity, ammonia concentration, and hydrogen sulfide concentration were measured after 15 days. The measurement results are shown in Table 2 below:

[0060] Table 2: Deodorizing effect of bacteria agent

[0061]

[0062] It can be seen from the above table that after 15 days of adding solid bacterial agents to pig manure, the odor intensity, ammonia concentration and hydrogen sulfide concentration were significantly lower than those of the control, of which the odor intensity decreased by 36.1%, the ammonia concentration decreased by 51.2%, and the hydrogen sulfide concentration decreased by 36.1%. Concentration decreased by 38.4%.

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Abstract

The invention relates to a livestock and poultry excrement microbial deodorizing agent, and a preparation method and an application thereof. The invention discloses a composite biological preparation used in public toilet deodorization. The composite biological preparation comprises a lactobacillus strain which is lactobacillus sp with a collection number of CGMCC NO: 5983, a lactobacillus strain which is streptococcus thermophilus with a collection number of CGMCC NO: 5985, and an orange peel extraction liquid. The invention also discloses the preparation method of the composite biological preparation. The method comprises preparation methods of a liquid composite biological preparation and a solid biological preparation. The invention also discloses a purpose of the composite biological preparation.

Description

technical field [0001] The invention relates to a microbial deodorant bacterial agent and a preparation method thereof, in particular to a microbial deodorant bacterial agent constructed based on the principles of microbial ecology by utilizing the interaction relations such as symbiosis, intergrowth, and synergistic effect between bacterial strains and a preparation method thereof . Background technique [0002] Odor pollution is one of the seven major environmental hazards recognized in the world. With the adjustment of my country's agricultural and rural economic structure, animal husbandry has achieved sustained, healthy and rapid development, but the odor pollution caused by livestock and poultry manure has become the most important contribution to odor pollution. One, especially along with the gradual improvement of economic development and people's living standards, the requirements for environmental quality are also becoming increasingly strong, and the stench produce...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/00C12N1/16C12N1/18A61L9/01C12R1/84C12R1/865A61L101/52
Inventor 闫志英刘晓风袁月祥廖银章李志东许力山
Owner CHENGDU INST OF BIOLOGY CHINESE ACAD OF S
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