Vitrification automatic operation system and operation method

Abstract

The invention discloses a vitrification automatic operation system and operation method; the system comprises a computer, an XY motor driving workbench, a CCD (Charge Coupled Device) camera, a vitrification device, an inverted microscope, a three degree-of-freedom automatic operator as well as a capillary tube clamper and a glass capillary tube, wherein the CCD camera is positioned below the XY motor driving workbench; the vitrification device is positioned on the XY motor driving workbench; the inverted microscope is positioned on the vitrification device; the three degree-of-freedom automatic operator is positioned beside the vitrification device; the capillary tube clamper and the glass capillary tube are arranged on the three degree-of-freedom automatic operator; and the vitrification automatic operation system further comprises a hydraulic pump, an operator driver and a stepping motor driver, wherein the hydraulic pump is connected with the computer and used for controlling the capillary tube clamper and the glass capillary tube; the operator driver is connected with the computer and used for driving the three degree-of-freedom automatic operator; and the stepping motor driver is connected with the computer and used for driving the XY motor driving workbench. According to the utility model, as manual operation is replaced with the vitrification automatic operation system and operation method, the process is centralized, the complicatedness in operation is reduced and the working efficiency can be greatly increased.

Description

technical field [0001] The invention relates to the technical field of vitrification, in particular to a vitrification automatic operating system and operation method. Background technique [0002] The cryopreservation of embryos or cells is an important part of human assisted reproductive technology. The traditional freezing method takes a long time, the equipment is expensive, and ice crystals may be formed during the freezing process, causing damage to cells. People have invented glass in the process of continuous exploration and research. Thaw freezing technology. [0003] Vitrification technology is a method of rapidly freezing cells or tissues, which can avoid the formation of ice crystals in the cells during the freezing and thawing process and minimize cell damage. The method is simple and the equipment is simple. It has been obtained in the cryopreservation of human cells and embryos. A lot of progress. In recent years, many scholars and experts have devoted thems...

AI Technical Summary

Problems solved by technology

When people work manually, problems such as nervousness, hand tremor, vision illusion, and fatigue may occur, and operational errors such as losing embryos or cells may occur, so that the stability, timeliness, and safety of the operation cannot be guaranteed.

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