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Immobilized lipase catalyst for preparing biodiesel and application of immobilized lipase catalyst

A lipase catalyst and a technology for biodiesel, which are applied to the preparation method thereof and the biodiesel prepared by using the same, the lipase catalyst immobilized on the sepiolite, and the lipase catalyst immobilized on sepiolite, can solve the problem of low reuse times and enzyme reaction. Harsh conditions, expensive enzymes, etc., to achieve the effect of stable activity, flexible modification, and improved high temperature resistance

Inactive Publication Date: 2012-12-19
连云港力润粮油贸易有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The reaction conditions of enzymatic preparation of biodiesel are mild, but the cost is high due to the high price of enzymes; and the reaction conditions of enzymes are harsh, easy to inactivate, and the number of times of reuse is low

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0072] A preparation method for an immobilized lipase catalyst, comprising the steps of:

[0073] (1) Hydrochloric acid modified sepiolite:

[0074] a. Add 100mL of hydrochloric acid with a concentration of 0.4mol / L to 10g of sepiolite at 30°C and soak for 12h;

[0075] b. Washing with distilled water, filtering, drying, pulverizing, and burning at 240° C. to obtain hydrochloric acid-modified sepiolite.

[0076] (2) Immobilization of lipase:

[0077] a. Dissolve 400mg lipase in 50mL phosphate buffer solution (0.05mol / L, pH7), centrifuge (2400r / min), take 100mL enzyme solution; add 4g of sepiolite carrier modified in step (1) ; shake the mixture at room temperature; after reacting for 6 hours, wash and filter with a large amount of buffer solution, and obtain immobilized lipase after drying.

[0078] Preparation of biodiesel using the prepared immobilized lipase catalyst:

[0079] (3) In a 0.25L reactor, add soybean oil and methanol (molar ratio 1:3), methanol is added thre...

Embodiment 2

[0081] A preparation method for an immobilized lipase catalyst, comprising the steps of:

[0082] (1) Hydrochloric acid modified sepiolite:

[0083] a. Add 150mL of hydrochloric acid with a concentration of 0.4mol / L to 10g of sepiolite at a temperature of 30°C and soak for 12h;

[0084] b. Washing with distilled water, filtering, drying, pulverizing, and burning at 240° C. to obtain hydrochloric acid-modified sepiolite.

[0085] (2) Immobilization of lipase:

[0086] Dissolve 400mg of lipase in 50mL of phosphate buffer solution (0.05mol / L, pH7), centrifuge (2400r / min), take 100mL of enzyme solution, add 4g of the modified sepiolite carrier in step (1); The mixture was shaken at room temperature; after reacting for 6 hours, the filter was washed with a large amount of buffer solution, and the immobilized lipase was obtained after drying.

[0087] Preparation of biodiesel using the prepared immobilized lipase catalyst:

[0088] (3) In a 0.25L reactor, add rapeseed oil and me...

Embodiment 3

[0090] A preparation method for an immobilized lipase catalyst, comprising the steps of:

[0091] (1) Hydrochloric acid modified sepiolite:

[0092] a. Add 200mL of hydrochloric acid with a concentration of 1.2mol / L to 10g of sepiolite at a temperature of 50°C and soak for 12h;

[0093] b. Washing with distilled water, filtering, drying, pulverizing, and burning at 240° C. to obtain hydrochloric acid-modified sepiolite.

[0094] (2) Immobilization of lipase:

[0095] Dissolve 400mg of lipase in 50mL of phosphate buffer solution (0.05mol / L, pH7), centrifuge (2400r / min), take 100mL of enzyme solution, add 4g of the modified sepiolite carrier in step (1); The mixture was shaken at room temperature; after reacting for 6 hours, the filter was washed with a large amount of buffer solution, and the immobilized lipase was obtained after drying.

[0096] Preparation of biodiesel using the prepared immobilized lipase catalyst:

[0097] (3) In a 0.25L reactor, add castor oil and meth...

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PUM

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Abstract

The invention discloses a lipase catalyst immobilized on sepiolite, a preparation method of the lipase catalyst and a method for preparing biodiesel from the catalyst, and belongs to the field of preparation of biodiesel. Dosage of the immobilized lipase catalyst is low, and the immobilized lipase catalyst can be reused by 10 times. In addition, the biodiesel prepared from the catalyst is high in reaction activity and high in reaction speed, reaction conditions are mild relatively, reaction can be carried out under atmospheric pressure at low temperature, and reaction energy consumption and cost can be reduced. Besides, a production process is environment-friendly, an operation process is simple, and erosion to equipment is low.

Description

technical field [0001] The present invention relates to a kind of immobilized lipase catalyst for preparing biodiesel, its preparation method and the method of using it to prepare biodiesel, in particular to a kind of lipase catalyst immobilized on sepiolite, its preparation method and its utilization The invention discloses a method for preparing biodiesel with a catalyst, belonging to the field of biodiesel preparation. Background technique [0002] According to the definition given by the National Biodiesel Borad (NBB) in 1992, biodiesel refers to clean alternative fuels produced from renewable biological resources such as plants and animal fats that can be used in compression ignition engines. With the gradual depletion of petroleum resources and the deterioration of the environment, biodiesel, as a substitute for petroleum fuels, has basically no sulfur and aromatics, high energy density, high cetane number, good lubrication performance, safe storage and transportation,...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N11/14C12P7/64
CPCY02E50/13Y02E50/10Y02P20/50
Inventor 李为民浦国佳李建防李志鹏张湘辉
Owner 连云港力润粮油贸易有限公司
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