Cell microscopic observation monitor in cell factory

Abstract

The invention relates to a cell microscopic observation monitor in a cell factory, belonging to the technical field of bioengineering. The cell microscopic observation monitor is characterized in that a stepping motor is used to control a sample introduction driving system, so that cell culture dishes can simultaneously enter an illuminating system part and an imaging system part of the monitor; an LED (light-emitting diode) lamp is used as the illuminating system to illuminate the cell culture dishes, and cells are imaged on a CCD (charge-coupled device) through a microscopic lens with a long working distance; and cell images obtained after CCD imaging are subjected to image amplification processing through an image acquisition card and an image processing apparatus so as to obtain relatively perfect images, and the images are presented on a PC (personal computer) display. The cell microscopic observation monitor is quick and convenient to operate and low in cost, realizes automatic operation, and uses a cell factory microscopic monitoring system with a long working distance to realize the quick observation monitoring of cells in the multi-layer culture dishes in the cell factory. The cell microscopic observation monitor is widely used for cell real-time monitoring in the field of biopharmaceutical industry.

Description

technical field [0001] The invention belongs to the technical field of bioengineering and relates to an improvement of a cell culture monitoring technology. Background technique [0002] In recent years, with the development of science and technology, especially the rapid development of cell culture in the field of biopharmaceuticals, the cell factory is a widely used large-scale cell culture container, which is composed of multi-layer culture dishes, so it is necessary to monitor and observe the cell factory Various microscopic instruments continue to appear, but they can only observe and monitor a single culture dish in real time. At present, there is no report on a real-time, fast, low-cost, automated and simple microscopic monitoring system for large-scale cultivation of multi-layer cell culture dishes in cell factories in China. . Modern biopharmaceutical and vaccine production enterprises urgently need a fast, low-cost, automated, and easy-to-operate real-time cell mo...

AI Technical Summary

Problems solved by technology

[0002] In recent years, with the development of science and technology, especially the rapid development of cell culture in the field of biopharmaceuticals, the cell factory is a widely used large-scale cell culture container, which is composed of multi-layer culture dishes, so it is necessary to monitor and observe the cell factory Various microscopic instruments continue to appear, but they can only observe and monitor a single culture dish in real time. At present, there is no report on a real-time, fast, low-cost, automated and simple microscopic monitoring system for large-scale cultivation of multi-layer cell culture dishes in cell factories in China.

Images