Method for efficiently expressing foreign protein

Abstract

The invention discloses a method for efficiently expressing foreign protein. The method comprises the steps of cloning a foreign protein gene onto a pMYC2 carrier, enabling the foreign protein gene and an encoding genes of six MYC tags on the pMYC2 carrier to form a fused gene, and then utilizing an agrobacterium infection transformation method to express the foreign protein in tobacco leaves instantly and efficiently; utilizing affinity media carried with corresponding tag antibodies to efficiently purify the protein expressed by tobacco according to MYC affinity tags carried by recombinant protein and finally performing competitive elution of the affinity media to obtain purified target protein. The purified protein can be used for further biological function research. The method achieves protein purification in one step and is simple and quick, and the protein purity is high. The foreign recombinant protein can be used for biological safety evaluation and biological function research.

Description

technical field [0001] The invention relates to a method for highly expressing foreign protein. Background technique [0002] The use of transgenic technology to produce high value-added proteins in bioreactors has important theoretical significance and application value. Bioreactors have gone through four stages: bacterial genetic engineering, cell genetic engineering, transgenic animal bioreactors and transgenic plant bioreactors. [0003] Bacterial genetic engineering products are often not biologically active, and must undergo a series of modifications such as glycosylation and hydroxylation before they can become effective drugs. Cell genetic engineering is also expensive because of the harsh culture conditions of mammalian cells. , which limits scale production. [0004] Animal bioreactor has the characteristics of high product quality and easy purification, which makes up for the defects of other various gene expression systems, but its technology itself involves mo...

AI Technical Summary

Problems solved by technology

However, in the transgenic plant reactor, the uncertainty of the insertion site of the exogenous gene and the complex interaction between genes often occur, which lead to a series of changes in the metabolism, development, and genetics of the transgenic crop, resulting in the so-called Unintended effects of GM crops

This effect is one of the important contents of the safety evaluation of genetically modified crops, but it is often difficult to accurately evaluate it with conventional analysis and detection methods.

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