Paraffin texture chip preparation method

Abstract

The invention discloses a paraffin texture chip preparation method which is a rapid, simple, economic and applicable preparation method, particularly aiming at the preparation of a texture microchip of which the diameter is less than 0.5mm; and the preparation method is precise and reliable. The preparation method specifically comprises the steps of punching holes and positioning, using a needle wire attached gel arranging isolating method, carrying out paraffin embedding, cooling, taking down the needle by using a release agent, and performing refusion so as to obtain the texture microchip. The paraffin texture chip prepared by using the preparation method has the advantages of being tidy in arrangement, uniform in interval, and high in chip preparation rate after being sliced, economic, practical, and low in cost, and cells are clear and visible without damage after being dyed, so that the texture microchip technology can be popularized and used more widely.

Description

technical field [0001] The invention relates to the field of tissue chip preparation, in particular to a method for making a paraffin tissue chip. Background technique [0002] Tissue microchip technology is a miniature biochemical analysis system that integrates the discontinuous analysis process of life science on the surface of the glass chip according to its biological characteristics, so as to realize the analysis of cells, proteins, genes and other organisms. Accurate, rapid, and large-information detection and screening of components. [0003] Biological microchips are a revolutionary tool for new drug discovery. In view of the rules of new drug R&D and marketing, once a new drug is finally approved by the FDA, it must be screened from the initial 10,000 compounds. Only 1% of the compounds may enter animal experiments, and only 5-3 of them may enter human trials. In the end, only one was approved for marketing. [0004] The research and development costs before an ...

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Problems solved by technology

[0008] In view of the above-mentioned deficiencies in the prior art, the purpose of the present invention is to provide a method for producing a paraffin tissue chip, which aims to solve the problems of complex process, low operation efficiency, and low slice yield after slicing in the prior art.

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