Culture medium for culturing bacillus subtilis for feedstuffs
A technology of Bacillus subtilis and culture medium, applied in the direction of bacteria, microorganism-based methods, microorganisms, etc., to achieve the effect of solving polluted environment, high production cost, and reducing culture cost
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example 1
[0023] Accurately weigh 10 grams of glucose, 5 grams of sodium chloride, potassium dihydrogen phosphate 3 and 1.5 grams of magnesium sulfate, put them in a beaker, and then weigh 20 grams of fishmeal wastewater concentrate, and then add 800 grams of water to the beaker and stir evenly , Heating, after the above components are completely dissolved, adjust the pH to 7.0 with 5mol / L NaOH, and add water to 1000 ml. Divide the prepared medium into 250 ml Erlenmeyer flasks, each with a volume of 60 ml, seal with a breathable sealing film, and autoclave at 121.3°C for 30 minutes.
example 2
[0025] Accurately weigh 10 grams of glucose, 5 grams of sodium chloride, potassium dihydrogen phosphate 3 and 1.5 grams of magnesium sulfate, put them into a beaker, and then weigh 50 grams of fishmeal wastewater concentrate, and then add 800 grams of water to the beaker and stir evenly , Heating, after the above components are completely dissolved, adjust the pH to 7.0 with 1mol / L NaOH, and add water to 1000 ml. Divide the prepared medium into 250 ml Erlenmeyer flasks, each with a volume of 60 ml, seal with a breathable sealing film, and autoclave at 121.3°C for 30 minutes.
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