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Method for extracting anti-tumor active component from traditional Chinese medicine pericarpium juglantis

An anti-tumor activity, Qinglong technology

Inactive Publication Date: 2012-11-21
LANZHOU INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, traditional Chinese medicine has the characteristics of integrity and multi-target action, and only the activity evaluation of a monomer compound in Qinglongyi cannot fully reflect the anti-tumor activity of traditional Chinese medicine Qinglongyi

Method used

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  • Method for extracting anti-tumor active component from traditional Chinese medicine pericarpium juglantis
  • Method for extracting anti-tumor active component from traditional Chinese medicine pericarpium juglantis
  • Method for extracting anti-tumor active component from traditional Chinese medicine pericarpium juglantis

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Preparation of active components of traditional Chinese medicine Qinglongyi:

[0022] Material source: Qinglongyi, a traditional Chinese medicine, is the immature dry exocarp of Juglans regia L., which was collected in the suburb of Tianshui City, Gansu Province in August.

[0023] Extraction and preparation: 1 kg of dried Qinglongyi, 5L of 70% ethanol was heated and refluxed at 80°C for 3 times, each time for 2 hours, filtered, combined for 3 extractions, concentrated (vacuum-0.07MPa, temperature 50°C), reduced Dry under pressure (vacuum-0.07MPa, temperature 50°C) to obtain extract I. After dispersing the extract I with 20 times the amount of water, extract 3 times with 2 times the amount of ethyl acetate of the extract I solution, combine the ethyl acetate extracts, concentrate, and dry under reduced pressure (vacuum -0.07MPa, temperature 50°C) , to obtain extract II. Extraction II is dissolved with 10 times the amount of 20% ethanol, the supernatant is taken, and t...

Embodiment 2

[0025] inhibit mouse S 180 and H 22 Assay for Growth Activity of Transplanted Tumors

[0026] Test animals: Kunming mice, male, weighing 18-22 g.

[0027] Drugs and reagents: physiological saline for injection (blank control), cyclophosphamide (positive control), active components of Qinglongyi. Tumor strain: mouse solid tumor S 180 and mouse hepatocarcinoma H 22 .

[0028] experiment method:

[0029] Effects on tumor weight and immune organ index in mice: Male mice were randomly divided into Qinglongyi active group, cyclophosphamide group and normal saline group. Tumor cell suspension (approximately 1×10 6 ~2×10 6 cell / mL) 0.2 mL was inoculated under the skin of the right anterior armpit of the mice, and 24 hours after the inoculation, each group was intragastrically administered (i.g.) for 10 consecutive days. After the last administration, the mice were killed, and the body weight, tumor weight, thymus and spleen mass were weighed, and the tumor inhibition rate, th...

Embodiment 3

[0039] Experiment of Inhibiting Proliferation Activity of Human Lung Cancer A549 Cell Line

[0040] Cell culture:

[0041]Human lung adenocarcinoma A549 cells were cultured in RPMI 1640 medium, plus 10% FBS. Take the cell count in the logarithmic growth phase, and take 2.5×10 4 / mL concentration in 96-well plate, 200 μL per well, in 5% CO 2 , 95%O 2 , 37°C, cultured in a saturated humidity cell incubator for 24 hours until the cells adhered to the wall.

[0042] Cytotoxicity Assay:

[0043] After the drug was dissolved in DMSO, it was diluted to different concentrations (3.125-100 μg / mL) with 1640 culture medium. 2 , 95%O 2 , culture in a saturated humidity incubator at 37°C for 48 hours, discard the cell solution, add 200 μL of 1640 culture solution and 20 μL of 5% MTT to each well and continue to incubate for 4 hours, discard the cell solution, add 150 μL DMSO to each well, and shake for 10 minutes to make the crystals fully After dissolving, use a microplate reader t...

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Abstract

The invention discloses a method for extracting an anti-tumor active component from traditional Chinese medicine pericarpium juglantis. The method uses a macroporous resin technology to extract the active component from the traditional Chinese medicine pericarpium juglantis. The component simultaneously has the activity of inhibiting growth of S180 and H22 mice transplanted tumors and inhibiting the proliferation of human lung cancer cell strain A549.

Description

technical field [0001] The invention relates to a method for extracting an active component of a traditional Chinese medicine, in particular to a method for extracting a component with antitumor activity from the traditional Chinese medicine Qinglongyi. Background technique [0002] The traditional Chinese medicine Qinglongyi is the immature exocarp of Jugland mandshurica Maxim. and Juglans regia L. . It was first recorded in "Kaibao Materia Medica", which is called walnut green dragon. It is recorded in the ancient books "Fangmai Authentic", "Emergency Prescription" and "Compendium of Materia Medica", and it is mainly used to treat stomach, abdominal pain, dysentery, painful swelling and sores, and skin sores. On the basis of inheriting the clinical experience of the predecessors, modern medical workers continue to conduct in-depth research, discover new medicinal effects of Qinglongyi, and broaden its medicinal scope. In terms of anti-tumor effect, the crude extract of t...

Claims

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Application Information

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IPC IPC(8): A61K36/52A61P35/00A61K131/00
Inventor 邸多隆孙小明柳军玺
Owner LANZHOU INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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