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Application of polysaccharide sulphate to preparation of tumor treatment neovascularization inhibitors and vascular disrupting agents

A technology of sodium alginate and new angiogenesis, which is applied in the direction of antineoplastic drugs and drug combinations, can solve the problems that there is no sodium alginate, etc., and achieve the effect of expanding the scope of application

Inactive Publication Date: 2012-11-21
OCEAN UNIV OF CHINA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

But at present, there is no report of sodium alginate as a drug for the treatment of tumors.

Method used

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  • Application of polysaccharide sulphate to preparation of tumor treatment neovascularization inhibitors and vascular disrupting agents
  • Application of polysaccharide sulphate to preparation of tumor treatment neovascularization inhibitors and vascular disrupting agents
  • Application of polysaccharide sulphate to preparation of tumor treatment neovascularization inhibitors and vascular disrupting agents

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Example 1: Inhibitory effect of sodium alginate diester on neovascularization of human umbilical vein endothelial cells

[0026] Experimental method: at 37°C, 5% CO 2 Human umbilical vein endothelial cells (HUVECs) were cultured in F-12 medium containing 10% FBS, 100IU / mL penicillin in an incubator. Pre-cool the 200 μL tip and 96-well plate at -20°C. Add 55 μL / well of Matrigel (matrigel) into a pre-cooled 96-well plate with a pre-cooled pipette tip, and then put the 96-well plate in a 37°C incubator for 30 minutes to solidify the Matrigel. Human umbilical vein endothelial cell suspension (9×10 4 cells / mL) were inoculated into solidified Matrigel wells, and after the drug was allowed to act for 7 hours, a uniform field of view was selected and photographed under a microscope (Olympus, Japan, ×100), and the experiment was repeated three times. A ring structure observed under the microscope is a lumen, and the inhibition rate of the formation of the lumen is calcula...

Embodiment 2

[0028] Example 2: Destructive effect of sodium alginate diester on mature blood vessels of human umbilical vein endothelial cells

[0029] Experimental method: Put Matrigel on ice and let it melt, while the pipette tip and 96-well plate are pre-cooled at -20°C. Add 55 μL / well of Matrigel with a pre-cooled tip into a pre-cooled 96-well plate, and then place the 96-well plate in a 37°C incubator for 30 minutes to solidify the Matrigel. Human umbilical vein endothelial cells (9×10 4 cells / mL) were inoculated into solidified Matrigel wells, cultured at 37°C for 6h to form a mature lumen, and then added different concentrations of sodium alginate diester 12.5, 25, 37.5, 50, 100μg / mL for 0.5h and 1h respectively , 3h, 5h, 7h, the same field of view was selected to take pictures at each time point (Olympus, Japan, ×40), and the experiment was repeated 3 times. A ring structure observed under a microscope is a lumen, and the destruction rate of the lumen formation is calculated ...

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Abstract

The invention provides application of polysaccharide sulphate to preparation of tumor treatment neovascularization inhibitors and vascular disrupting agents. Experiments prove that the neovascularization inhibiting rate, the maturation blood vessel disrupting rate and the concentration of the polysaccharide sulphate are in dosage relying relationship in a certain range, and the maturation blood vessel disrupting rate and the polysaccharide sulphate acting time are in dosage relying relationship. The application provided by the invention has the advantages that the effect of the polysaccharide sulphate in the aspects of tumor neovascularization inhibiting and tumor blood vessel generation disrupting can be proved, and the polysaccharide sulphate can be used for tumor blood vessel generation and other blood vessel generation disease treatment, and can also be used in aspects of tumor chemotherapy and / or auxiliary chemotherapy. Many diseases of people are relevant to the blood vessel generation, and the polysaccharide sulphate as the neovascularization inhibitors and vascular disrupting agents can be widely applied to the treatment of diseases of tumor and the like. The application provided by the invention has the advantages that the application range of the polysaccharide sulphate is widened, and the scientific basis is provided for the purpose development of the polysaccharide sulphate in the anti-tumor aspect and the like.

Description

technical field [0001] The invention relates to a new application of sodium alginate diester, in particular to an application of the diester sodium alginate in preparing an angiogenesis inhibitor and a blood vessel destroyer for treating tumors. Background technique [0002] Tumor is one of the most serious diseases that endanger human health. At present, there are many ways to treat cancer. Anti-angiogenesis therapy developed based on the relationship between angiogenesis and tumor growth and metastasis is a promising method. Treatment brings new hope. Angiogenesis is the creation of new blood vessels by sprouting or dividing existing blood vessels. Under physiological and pathological conditions, such as embryogenesis, female reproductive cycle, inflammatory response, wound healing, tumorigenesis and other processes, angiogenesis is carried out, especially under pathological conditions. According to statistics, about 20-40 human diseases are related to angiogenesis. rela...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/737A61P35/00A61K31/734
Inventor 邱培菊管华诗
Owner OCEAN UNIV OF CHINA
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