Rapid testing card for heavy metal mercury and testing method thereof

A detection method and technology for heavy metals, applied in the field of heavy metal detection, can solve the problems of poor selectivity, high sensitivity of enzymatic methods, difficult detection, etc., and achieve the effects of low cost, sensitive reaction and easy operation.

Active Publication Date: 2012-11-14
广东中检达元检测技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The enzymatic method has high sensitivity, but poor selectivity, and there are certain difficulties in the detection of a single heavy metal example....

Method used

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  • Rapid testing card for heavy metal mercury and testing method thereof
  • Rapid testing card for heavy metal mercury and testing method thereof
  • Rapid testing card for heavy metal mercury and testing method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Production of Heavy Metal Mercury Rapid Detection Card: Glue absorbent filter paper with a diameter of 15mm and absorbent filter paper with a diameter of 12mm to the bottom plate respectively, and after connecting the cover membrane, take 20 μL of color developer and add it to the absorbent filter paper (15mm in diameter) , add 10 μL of urease solution to another absorbent filter paper (diameter 12 mm), dehumidify at room temperature for 2 hours, cover with film, put in a packaging bag and seal, and store at 4-8 °C.

[0039] Among them, the chromogenic agent is composed of phenol red and urea mixed and dissolved in ethanol aqueous solution. Its formula is: 0.1% phenol red, 0.8% urea, 10% absolute ethanol, and the balance is water; 1.2 U / ml.

Embodiment 2

[0041] The making of the heavy metal mercury rapid detection card is the same as in Example 1, wherein, the developer formula: 0.1% phenol red, 0.8% urea, 15% dehydrated alcohol, and the balance is water; the urease liquid is green soybean urease liquid, and the concentration of the urease liquid is 1.2 U / mL.

Embodiment 3

[0043] The making of the heavy metal mercury rapid detection card is the same as in Example 1, wherein, the developer formula: 0.1% phenol red, 0.8% urea, 10% dehydrated alcohol, and the balance is water; the urease liquid is green soybean urease liquid, and the concentration of the urease liquid is 1.6 U / mL.

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PUM

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Abstract

The invention discloses a rapid testing card for heavy metal mercury and a testing method thereof. The structure of the testing card includes a base board, absorption wafers and a cover film successively from bottom to top. At least one wafer for absorbing color-developing agents and at least one wafer for absorbing urease are respectively fixed on the base board, and the wafers are not overlapped mutually. The use amount ratio of the urease and the color-developing agents absorbed by the wafer per unit area is 3U: (1.6-3.2mL), and the color-developing agents contain components of, by mass, 0.05% to 2.5% of phenol red and 0.8% to 2% of urea. The testing card can test the content of mercury larger than or equal to 0.2 mg/L rapidly, accurately and conveniently; the test card is low in cost, simple and convenient to operate, short in testing time, in no need of professionals, sensitive in reaction and easy to observe and interpret; and the testing card is non-toxic and harmless and cannot cause environment pollution.

Description

technical field [0001] The invention relates to heavy metal detection, in particular to a heavy metal mercury rapid detection card and a detection method thereof. Background technique [0002] With the development of industry, water quality and soil are seriously polluted by heavy metals, which will have adverse effects on human health. The current heavy metal detection needs to be digested by strong acid, high pressure, and high temperature samples, and then tested by atomic absorption instruments and other equipment. For heavy metal detection samples, there are many pre-treatment procedures, long detection cycles, professional equipment and professional operators are required, and waste liquids pollute the environment. The problem is that providing a method and / or product for rapid detection of heavy metals is very important to meet the current market demand for rapid on-site heavy metal detection and screening of a large number of samples, and to fill the gaps at home and...

Claims

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Application Information

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IPC IPC(8): C12Q1/58
Inventor 贺取华谢俊平卢新
Owner 广东中检达元检测技术有限公司
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