Organic garbage decomposing bacterium agent, as well as preparation method and application of organic garbage composting bacterium agent
A technology of organic waste and decomposed bacterial agents, which is applied in the preparation and application of organic fertilizers, organic fertilizers, etc., can solve problems such as pollution, and achieve the effects of simple process, shortened fermentation cycle, and reduced odor
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[0027] In the present embodiment, the preparation method of organic waste decomposing bacterial agent is as follows:
[0028] (1) Inoculate the corresponding bacteria of No. 1 composite bacterial agent and No. 2 composite bacterial agent in different mediums, and then shake and culture in a shaker at 30°C at 180r / min for 1 day. After the culture is completed, dilute to form a suitable concentration of bacterial liquid , the number of bacteria per milliliter is about 200 million, that is, 200 million / ml;
[0029] Photosynthetic bacteria culture medium is: NH 4 Cl 1.0g / L, K 2 HPO 4 0.5g / L, MgCl 2 0.2g / L, NaCl 2.0g / L, yeast extract 0.1g / L, pH 7.0.
[0030] Pseudomonas fluorescens medium: beef extract 3.0g / L, peptone 10.0g / L, NaCl 5.0g / L, pH 7.0-7.2.
[0031] The culture medium for Bacillus and Streptomyces violaceum is: yeast extract 2.0g / L, glucose 8.0g / L, K 2 HPO 4 6.5g / L, NaNO 3 3.0g / L, NH 4 Cl 1.0g / L, NaCl 3.0g / L, MgSO 4 0.05g / L, MnSO 4 0.01g / L, FeSO 4 ·7H ...
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[0044] Manufacturing example
[0045] In the present embodiment, the preparation method of organic waste decomposing bacterial agent is as follows:
[0046] (1) Inoculate the corresponding bacteria of No. 1 composite bacterial agent and No. 2 composite bacterial agent in different mediums, and then shake and culture in a shaker at 30°C at 180r / min for 2 days. After the culture is completed, dilute to form a suitable concentration of bacterial liquid , the number of bacteria per milliliter is about 300 million, that is, 300 million / ml;
[0047] Photosynthetic bacteria culture medium is: NH 4 Cl 1.0g / L, K 2 HPO 4 0.5g / L, MgCl 2 0.2g / L, NaCl 2.0g / L, yeast extract 0.1g / L, pH 7.0.
[0048] Pseudomonas fluorescens medium: beef extract 3.0g / L, peptone 10.0g / L, NaCl 5.0g / L, pH 7.0-7.2.
[0049] The culture medium for Bacillus and Streptomyces violaceum is: yeast extract 2.0g / L, glucose 8.0g / L, K 2 HPO 4 6.5g / L, NaNO 3 3.0g / L, NH 4 Cl 1.0g / L, NaCl 3.0g / L, MgSO 4 0.05g / L...
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