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Cryoprotectant and method for cryopreserving placenta amnion and chorion

A technology of cryoprotective liquid and chorion, applied in biochemical equipment and methods, artificial cell constructs, microorganisms, etc., can solve problems such as freezing damage and achieve the effect of preventing damage

Active Publication Date: 2014-04-02
BOYALIFE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although cryogenic freezing technology enables long-term preservation of engineered tissues, it also brings the problem of freezing injury
However, there is no report on low-temperature cryopreservation technology for human placental amnion and chorion

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Embodiment 1: Using the method of the present invention to freeze human placenta amnion and chorion

[0020] 1. Freezing method

[0021] Placenta with negative test results for infectious diseases such as hepatitis, syphilis, and AIDS and without obstetrical complications were selected, and the informed consent of the puerpera was obtained and signed an informed consent form before the operation. A sterile polystyrene storage bottle was prepared before the operation as a delivery bottle, which contained commercially available cell culture medium, and was sent to the cryopreservation place within 12 hours at 4°C after collection.

[0022] Under a sterile environment, gently separate the amnion and chorion with toothed forceps, and cut the amnion and chorion with surgical scissors. Rinse the amnion and chorion with normal saline for 2-3 times respectively, and after removing surface blood clots and other dirt, cut the amnion and chorion into about 1cm in a petri dish 2 ...

Embodiment 2

[0028] Embodiment 2: Using the method of the present invention to freeze human placenta amnion and chorion

[0029] 1. Freezing method

[0030] Placenta with negative test results for infectious diseases such as hepatitis, syphilis, and AIDS and without obstetrical complications were selected, and the informed consent of the puerpera was obtained and signed an informed consent form before the operation. A sterile polystyrene storage bottle was prepared before the operation as a delivery bottle, which contained commercially available cell culture medium, and was sent to the cryopreservation place within 12 hours at 4°C after collection.

[0031] Under a sterile environment, gently separate the amnion and chorion with toothed forceps, and cut the amnion and chorion with surgical scissors. Rinse the amnion and chorion with normal saline for 2-3 times respectively, and after removing surface blood clots and other dirt, cut the amnion and chorion into about 1cm in a petri dish 2 ...

Embodiment 3

[0037] Embodiment 3: Using the method of the present invention to freeze human placenta amnion and chorion

[0038] 1. Freezing method

[0039] Placenta with negative test results for infectious diseases such as hepatitis, syphilis, and AIDS and without obstetrical complications were selected, and the informed consent of the puerpera was obtained and signed an informed consent form before the operation. A sterile polystyrene storage bottle was prepared before the operation as a delivery bottle, which contained commercially available cell culture medium, and was sent to the cryopreservation place within 12 hours at 4°C after collection.

[0040] Under a sterile environment, gently separate the amnion and chorion with toothed forceps, and cut the amnion and chorion with surgical scissors. Rinse the amnion and chorion with normal saline for 2-3 times respectively, and after removing surface blood clots and other dirt, cut the amnion and chorion into about 1cm in a petri dish 2 ...

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PUM

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Abstract

The invention relates to the technical field of tissue engineering and discloses a cryoprotectant and a method for cryopreserving placenta amnion and chorion. The cryoprotectant is composed of fetal calf serum, dimethyl sulfoxide and dextran 40, and the volume ratio of the fetal calf serum, the dimethyl sulfoxide and the dextran 40 is 7-9:1:1. On the basis of deep research of placenta amnion and chorion structures, the dimethyl sulfoxide, the dextran 40 and the fetal calf serum suitable for protecting placenta amnion stem cells and chorion stem cells are selected to form the cryoprotectant. The fetal calf serum, the dimethyl sulfoxide and the dextran 40 coordinate to protect activity of cells, the cells are prevented from forming ice crystal and being damaged, and the activity of recovered stem cells and the activity of newly prepared amnion chorion stem cells have no difference.

Description

technical field [0001] The invention relates to the technical field of tissue engineering, in particular to a cryoprotectant solution and a method for cryopreserving human placenta amnion and chorion. Background technique [0002] Amnion and chorion are the two main components of the human placenta. The amniotic membrane is the innermost layer of the placenta, the membrane body is smooth, without blood vessels, nerves and lymph, and has a certain degree of elasticity. It is rich in pluripotent stem cells that are pluripotent and will not induce immune rejection after transplantation, human placental amnion cells It can also express nerve cell-specific markers, secrete neurotrophic factors, and be used for nervous system diseases. In addition, human placental amnion can also be used as the basement membrane of ophthalmic surgery; and the chorion is the main part of the placenta, adjacent to the amnion, on the chorion It also contains abundant stem cells with multi-differenti...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/071C12N5/00
Inventor 李春波吕康涛燕舞江鹤
Owner BOYALIFE
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