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Primers used for rapid detection of Neofusicoccum parvum and application thereof

A technology for grape canker bacteria and strains, which is applied to a primer for rapid detection of grape canker bacteria and its application field, can solve problems such as no molecular detection technology, and achieve the effects of shortening identification time, high sensitivity, and overcoming cumbersome steps.

Inactive Publication Date: 2012-10-24
BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES
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  • Abstract
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  • Application Information

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Problems solved by technology

However, in terms of detection of grape canker, there is no systematic research on molecular detection technology in China.

Method used

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  • Primers used for rapid detection of Neofusicoccum parvum and application thereof
  • Primers used for rapid detection of Neofusicoccum parvum and application thereof
  • Primers used for rapid detection of Neofusicoccum parvum and application thereof

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Embodiment 1

[0022] Example 1. Primers and applications thereof for rapid detection of grape canker bacterium Neofusicoccum parvum

[0023] 1. Acquisition of primers for rapid detection of grape canker bacterium Neofusicoccum parvum

[0024] The ITS sequences of 5 Neofusicoccum parvum sequenced by our laboratory were compared with the ITS sequences of 20 different species of Botrytis in GeneBank (accession numbers are: GU251147; HQ529768; JN088510; GU292676; FJ904819; EU683673; HQ288230; EF585518;JN088050;HQ625634;FJ888469;JN849098;JN035222;FJ904815;EU331085;DQ458889;HQ629954;AY236954;EU520175;AB454278)进行同源性比较,根据Neofusicoccum parvum与其它种间的差异位点设计Neofusicoccum parvum的特异性引物 , the resulting primer is the primer B.p-F / B.p-R of the present invention. The primer sequence is: upstream forward primer B.p-F sequence: 5'-AAAACTCCAGTCAGTGAACTTC-3' (sequence 1 in the sequence listing); downstream reverse primer B.p-R sequence: 5'-GGTCAACCTTGAGAAATAATTCAAAG-3' (sequence 2 in the sequence listing ).

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Abstract

The invention discloses a pair of primers used for rapid detection of Neofusicoccum parvum strains and application thereof. Nucleotide sequences of the pair of the primers are respectively represented by sequence 1 and sequence 2 in a sequence table, and the primers can specifically amplify the product of 370bp in Neofusicoccum parvum and have a sensitivity of 10 pg to genomic DNAs of the Neofusicoccum parvum strain. It is proved that the pair of the primers can be used for rapid reliable detection and identification of the Neofusicoccum parvum strain in disease samples of grapes in the field, and the problems of cumbersome steps, a long identification period and the like in traditional identification methods are overcome.

Description

technical field [0001] The invention relates to a pair of rapid molecular detection primers for grape canker bacterium Neofusicoccum parvum strain and application thereof. Background technique [0002] Grape canker (Grape Botryosphaeriaceae Canker) is a disease caused by the fungus infection of Botryosphaeriaceae (Botryosphaeriaceae). 14 countries including South Africa and China have occurred and caused serious losses. At present, it is known that 15 species of Botryosphaeriaceae can cause the disease (Auger J, Esterio M, Ricke G, et al. Black dead arm and basal canker of Vitis vinifera cv. Red Globe caused by Botryosphaeria obtusa in Chile [J].Plant Disease.2004,88(11):1286.Niekerk J M,Fourie P H,Hallenn F,et al.Botryosphaeria spp.as grapevine trunk disease pathogens[J].Phytopathologia Mediterranea.2006,45(4): 43-54.U Rbez-Torres J R,Peduto F,Striegler R K,et al.Characterization of fungal pathogens associated with grapevine trunk diseases in Arkansas and Missouri[J].Fungal...

Claims

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Application Information

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IPC IPC(8): C12N15/11C12Q1/68C12R1/645
Inventor 张玮李兴红燕继晔严红
Owner BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES
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