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Application of viable cells for improving skin appearance

A technology for improving skin and skin, applied in the directions of skin care preparations, skin diseases, cosmetic preparations, etc.

Inactive Publication Date: 2012-10-24
ENHANCE BIOMEDICAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] So far, these stem cells have not been used as active ingredients of compositions for the purpose of improving skin aging

Method used

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  • Application of viable cells for improving skin appearance
  • Application of viable cells for improving skin appearance
  • Application of viable cells for improving skin appearance

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] [Example 1] Experimental design was carried out as follows

[0032] Experimental groups were planned using the Latin square design (Kirk, 1995) experimental method. The problem of the sequence of experiments is dealt with by the principle of counterbalancing (Table 1).

Embodiment 2

[0033] [Example 2] Carry out pig breed captive breeding as follows

[0034] A total of one Lanyu boar (five months old) and three Lanyu sows (ages of six, seven, and nine years) were purchased. Each pig is accompanied by a pedigree registration certificate and production history. The pigs in captivity are provided with enough space for individual breeding. There are three areas for feed, rest, and manure. The concrete floor of the pig house has a raised strip floor, and the walls The edge is rich in automatic water supply device. There are no sharp protrusions in the pen, which is a non-closed breeding space, allowing pigs to see, smell, hear and contact the outside world. All experimental animals were raised with the least pathogen level management method, limited feed was provided twice a day, and medicines were rotated for cleaning and disinfection once or twice a month.

Embodiment 3

[0035] [Example 3] Peripheral blood stem cell collection was performed as follows

[0036]Peripheral blood stem cells are obtained through a blood separator. Five days before the separation, a dose of leukocyte growth hormone (GCSF) 300μg / 0.7ml (Filgrastim M300) was injected into the ear vein until the day before the final collection day, so as to rapidly release the hematopoietic stem cells in the bone marrow into the peripheral blood so that collect. Blood was drawn from jugular vein, shaken slowly and mixed with anticoagulant, centrifuged at 1800g for 15 minutes, then centrifuged at 700g for 5 minutes, and buffy coat cells rich in leukocytes were separated.

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PUM

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Abstract

A pharmaceutical composition for improving aged appearance of skin, comprises an effective dose of viable cell component. The viable cell component is chosen from the following group of: nucleated cells with surface antigen CD34 which come from bone marrow and peripheral blood pharmaceutically.

Description

technical field [0001] The present invention relates to a pharmaceutical composition for improving the appearance of aging skin and its application, in particular to a pharmaceutical composition for improving the aging appearance of human facial skin and its application. Background technique [0002] The pathways of skin aging are divided into two pathways: natural aging and photoaging (Fisher et al., 2002). The natural aging of the skin is mainly controlled by chromosomes. At present, medical technology cannot prevent it from changing chromosomes. Start by avoiding known aggravating factors (eg, exposure to certain hazardous substances). The two aging pathways can be distinguished by the parts covered by clothing and the parts often exposed to the sun, and the degree of skin aging is also different. A tendency to decrease keratinocyte stem cells can be observed in natural aging (Bosset et al., 2003; Kwon et al., 2008; Webb et al., 2004), and in microstructural aging such a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/14A61K38/39A61K8/98A61P17/00A61Q19/08A61K31/728A61K35/28
Inventor 韩鸿志林欣荣邱紫文林珀丞
Owner ENHANCE BIOMEDICAL
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