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Method for producing acetone butanol from fermented cassava

A kind of technology of acetone butanol and cassava, applied in the field of fermentation production of acetone butanol

Inactive Publication Date: 2012-10-17
INST OF PROCESS ENG CHINESE ACAD OF SCI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It is obviously unrealistic to continue to expand the production scale of chemical synthesis to meet the growing demand for acetone and butanol

Method used

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  • Method for producing acetone butanol from fermented cassava
  • Method for producing acetone butanol from fermented cassava
  • Method for producing acetone butanol from fermented cassava

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Embodiment 1: Clostridium acetobutylicum ATCC 824 utilizes cassava flour optimization medium fermentation to produce ABE

[0017] Prepare 200ml of cassava flour medium, medium composition: 7% cassava flour, boil and gelatinize the prepared cassava flour fermentation medium for 10min, transfer it to a 250ml shake flask, and autoclave at 121°C for 1.5h. To prepare the CSP-Fe solution, first prepare 100 mL of 20% corn steep liquor dry powder solution, add 0.1 g of ferrous sulfate heptahydrate, adjust the pH to 7.0 with sodium hydroxide, and autoclave at 121° C. for 20 minutes. When the temperature of the culture medium was lowered to below 40°C, different amounts of sterilized CSP-Fe solutions were added to the cassava flour, and then the activated Clostriidium acetobutylicum (Clostriidium acetobutylicum) ATCC 824 bacterial solution was inserted for 24 hours, and the inoculum size was 10 %, 37 ℃ fermentation culture.

[0018] After the fermentation, the fermentation broth...

Embodiment 2

[0030] Example 2: Fermentation of C.butylicum 23592 using cassava flour optimized medium to produce ABE

[0031] The cassava flour medium and CSP-Fe solution were prepared as described in Example 1. After the temperature of the medium was lowered to below 40°C, 2 mL of the sterilized CSP-Fe solution was added to the cassava flour, and then the activated C. Butylicum 23592 bacterial liquid, inoculum size 10%, fermented at 37°C.

[0032] After the fermentation, the fermentation broth was taken, the sample was centrifuged at 10000rpm for 10min, and the supernatant was collected. The ABE solvent components in the supernatant were analyzed by gas chromatography (GC).

[0033] The results showed that when no CSP-Fe solution was added, the rate and yield of ABE solvent produced by cassava flour fermented by C.butylicum 23592 bacterial liquid were low, and the total solvent concentration at the end of fermentation was 9.26g / L; After 2mL of CSP-Fe mixed solution, the total solvent co...

Embodiment 3

[0034] Example 3: C.acetobutylicum 170 uses cassava flour to optimize medium fermentation to produce ABE

[0035] The cassava flour medium and CSP-Fe solution were prepared as described in Example 1. After the temperature of the medium was lowered to below 40°C, 2 mL of the sterilized CSP-Fe solution was added to the cassava flour, and then the activated C. Acetobutylicum 170 bacteria liquid, 10% inoculum, fermented at 37°C.

[0036] After the fermentation, the fermentation broth was taken, the sample was centrifuged at 10000rpm for 10min, and the supernatant was collected. The ABE solvent components in the supernatant were analyzed by gas chromatography (GC). The results showed that when no CSP-Fe solution was added, the rate and yield of ABE solvent produced by cassava flour fermented by C.acetobutylicum 170 bacteria liquid were low, and the total solvent concentration at the end of fermentation was 10.18g / L; After 2mL of CSP-Fe mixed solution, the total solvent concentrat...

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Abstract

The invention relates to a method for producing acetone butanol by the utilization of cassava fermentation. The method comprises the following concrete technological steps of: preparing a medium by using a cassava powder as a raw material, boiling and gelatinizing for 90 minutes, preparing a mixed solution of a dried corn steep liquor powder and ferrous sulphate, adjusting pH of the solution, sterilizing, adding into cassava jelly, reducing the temperature to 37 DEG C, inoculating, followed by fermentation culture to obtain an acetone butanol fermentation broth. The invention has the following characteristics: first, original technological equipment can be reasonably utilized to produce acetone butanol at large scale without reconstruction of the equipment; second, the utilization rate of the strain for the cassava raw material is remarkably raised, the raw material cost for the production of acetone butanol by a fermentation method is reduced, and the economic benefit of acetone butanol production is raised; and finally, the cassava resource is fully utilized so as to drive the development of rural economy, increase incomes of peasants and produce profound social benefits.

Description

field of invention [0001] The invention relates to a method for producing acetone-butanol by fermentation, in particular to a method for adding cassava into cassava to facilitate the growth of bacteria so as to effectively utilize cassava to ferment and produce acetone-butanol. Background technique [0002] With the increasing shortage of oil resources in the world, countries all over the world are actively looking for the development and utilization of renewable resources. As an important renewable energy, biofuel has attracted the attention of various countries. It is estimated that the proportion of biofuel will increase to 30% by 2020, and the total demand will reach 87 billion gallons. [0003] At present, butanol and acetone have become the most important basic chemical bulk products in the world. The annual sales volume of butanol is close to 4 million tons, and the annual sales volume of acetone has exceeded 6 million tons, and the demand is still increasing. At pre...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/28C12P7/16C12P7/06C12R1/145
CPCY02E50/17Y02E50/10
Inventor 万印华李敬张超薛宏光齐本坤苏仪
Owner INST OF PROCESS ENG CHINESE ACAD OF SCI
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