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Hevea brasiliensis latex small G-protein Rop family protein and coding gene thereof

A technology encoding genes and proteins, applied in genetic engineering, plant genetic improvement, angiosperms/flowering plants, etc., can solve the problems of no more in-depth research, no cloned member genes, etc., and achieve the effect of improving resistance and increasing yield

Inactive Publication Date: 2012-10-17
RUBBER RES INST CHINESE ACADEMY OF TROPICAL AGRI SCI
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  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

(Li, D., et al., Identification and characterization of genes associated with tapping panel dryness from Hevea brasiliensis latex using suppression subtractive hybridization. BMC Plant Biol, 2010.10: p.140), they only reported the partial sequence of the Rop member, The member gene was not cloned nor further studied

Method used

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  • Hevea brasiliensis latex small G-protein Rop family protein and coding gene thereof
  • Hevea brasiliensis latex small G-protein Rop family protein and coding gene thereof
  • Hevea brasiliensis latex small G-protein Rop family protein and coding gene thereof

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Embodiment 1

[0034] Example 1. Acquisition of the gene encoding the small G protein Rop family member protein HbRop3 and its effect verification

[0035] For the first time, we cloned the full-length cDNA of the protein coding gene HbRop3, a member of the small G protein Rop family in Hevea latex, and conducted sequence analysis and functional studies.

[0036] 1. Acquisition of the small G protein Rop family member protein coding gene HbRop3

[0037]We established the Hevea latex EST sequence database and found the EST sequence of the small G protein Rop member coding gene HbRop3. According to Gentyx software analysis, we obtained the cDNA sequence with a complete reading frame. Therefore, we only need to design primers for verification again. The specific steps are as follows:

[0038] HbRop3 cDNA full-length clone

[0039] Design a primer at the 5' end and 3' end according to the EST sequence (3-F (sense strand primer): 5'-TCATTGCTCTGTCTATGTTGCTCA-3'; 5-1009R (antisense strand primer)...

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Abstract

The invention discloses a small G-protein Rop family protein, a coding gene and an application of the hevea brasiliensis latex small G-protein Rop family protein. The protein is a protein having one of the following amino acid residue sequences: 1) protein composed of an amino acid residue sequence of SEQ ID No.1 in a sequence table; 2) SEQ ID No.1 derived protein obtained by subjecting the amino acid residue sequence of SEQ ID No.1 in the sequence table to substitution and / or deletion and / or addition of one or more amino acid residues and having GTP (guanosine triphosphate) binding function. The protein has GTP binding activity, plays a certain role in basic disease-resistant mechanism and stress resistance of plants, and has the characteristics of control for secondary xylem and phloem development of woods. The protein can improve the resistance of transgenic crop or woods against adverse situation, so as to improve the yield.

Description

technical field [0001] The invention relates to a member protein HbRop3 of the rubber tree latex small G protein Rop family and the application of the coding gene thereof. Background technique [0002] The small G protein is a monomeric protein that binds to guanylic acid (GTP), and acts as a molecular switch in eukaryotic cells (Zhenbiao Yang, Small GTPases: Versatile Signaling Switches in Plants. The Plant Cell, 2002.14, p.S375-S388 ; V Vernoud, V., et al., Analysis of the small GTPase gene superfamily of Arabidopsis. Plant physiology, 2003.131 (3): p.1191-1208; Nibaua, C., Wu, HM. and Cheung, AY., RAC / ROP GTPases: 'hubs' for signal integration and diversification in plants. Trends in Plant Science, 11(6):p.309-315). Many studies have confirmed that members of the plant small G protein Rop subfamily are a unique class of genes formed in plant evolution, and they are widely involved in various physiological activities in plant cells, including the organization of actin cyt...

Claims

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Application Information

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IPC IPC(8): C07K14/415C12N15/29C12N15/63C12N5/10C12N1/21A01H5/00
Inventor 秦云霞戚继艳黄亚成王岳坤龙翔宇阳江华唐朝荣
Owner RUBBER RES INST CHINESE ACADEMY OF TROPICAL AGRI SCI
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